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GAPDH Rabbit pAb (AC001)

Review (1)Publications (446) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:GAPDH Rabbit pAb
ABclonal:Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of PC-12 cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of HeLa cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - GAPDH Rabbit pAb (AC001)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg GAPDH antibody (AC001). Western blot was performed from the immunoprecipitate using GAPDH antibody (AC001) at a dilution of 1:20000.

Review
ABclonal: review for GAPDH Rabbit pAb(AC001)

Overview

Product nameGAPDH Rabbit pAb
Catalog No.AC001
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternativ
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
SequenceMGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID
Swiss Prot
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW36kDa
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:30000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, Hep G2, Mouse liver, Rat liver
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region.
Customer validation

(MSC cells)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Gallus gallus, Danio rerio, Ctenopharyngodon idellus, Paralichthysolivaceus, Cyprinus carpio, Cobitis taenia Linnaeus, Ovis aries, Mus musculus, Cryptosporidium parvum, Chicken, Capra hircus, Other, Bos taurus, Rattus norvegicus, Misgurnus anguillicaudatus, Oryctolagus cuniculus)

Co-IP(Arabidopsis thaliana)

ELISA(Mus musculus)

Co-IP(Homo sapiens)

IHC(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus)

IHC(Rattus norvegicus, Mus musculus)

WB(Rattus norvegicus, Gallus gallus)

IF(Homo sapiens)

IF(Mus musculus, Rattus norvegicus, Gallus gallus)

RT-qPCR(Mus musculus)

qPCR(Homo sapiens)

RT-qPCR(Homo sapiens, Gallus gallus, Mus musculus)

Other(Other)

IP(Gallus gallus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - GAPDH Rabbit pAb (AC001)}

Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - GAPDH Rabbit pAb (AC001)}

Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)}

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)}

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)}

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)}

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)}

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)}

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of PC-12 cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)}

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)}

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of HeLa cells using GAPDH Rabbit pAb(AC001) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - GAPDH Rabbit pAb (AC001)}

Immunoprecipitation - GAPDH Rabbit pAb (AC001)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg GAPDH antibody (AC001). Western blot was performed from the immunoprecipitate using GAPDH antibody (AC001) at a dilution of 1:20000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (7)

GAPDH Mouse mAb
AC002

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

GAPDH Mouse mAb

GAPDH Rabbit mAb(High Dilution)
A19056

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

GAPDH Rabbit mAb(High Dilution)

GAPDH Mouse mAb (High Dilution)
AC033

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

GAPDH Mouse mAb (High Dilution)

GAPDH Rabbit pAb
AC027

WBIHC-PIF/ICCIP

Reactivity:Human, Mouse, Rat

GAPDH Rabbit pAb

GAPDH (Human Specific) Rabbit mAb
AC036

ELISAWBIF/ICC

Reactivity:Human

GAPDH (Human Specific) Rabbit mAb

HRP-conjugated GAPDH Mouse mAb
AC035

ELISAWB

Reactivity:Human, Mouse, Rat

HRP-conjugated GAPDH Mouse mAb

HRP-conjugated GAPDH Rabbit mAb
AC054

ELISAWB

Reactivity:Human, Mouse, Rat

HRP-conjugated GAPDH Rabbit mAb

Secondary Antibodies (25)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb