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GAPDH Rabbit pAb (AC027)

Publications (50) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:GAPDH Rabbit pAb
ABclonal:Western blot - GAPDH Rabbit pAb (AC027)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC027) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC027)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC027)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC027)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC027)

Immunofluorescence analysis of C6 cells using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC027)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC027)

Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGAPDH Rabbit pAb
Catalog No.AC027
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
SequenceMGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID
Swiss Prot
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, HepG2, Mouse liver, Mouse kidney, Rat liver, Rat kidney
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region.
Customer validation

WB(Homo sapiens, Mus musculus, Danio rerio, Ovis aries, Oryctolagus cuniculus, Ctenopharyngodon idellus, Mus musculus, Rattus norvegicus, Other)

IF(Homo sapiens)

IP(Homo sapiens)

IF(Homo sapiens)

Other(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - GAPDH Rabbit pAb (AC027)}

Western blot - GAPDH Rabbit pAb (AC027)

Western blot analysis of various lysates using GAPDH Rabbit pAb (AC027) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC027)}

Immunohistochemistry - GAPDH Rabbit pAb (AC027)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC027)}

Immunohistochemistry - GAPDH Rabbit pAb (AC027)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC027)}

Immunohistochemistry - GAPDH Rabbit pAb (AC027)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC027)}

Immunofluorescence - GAPDH Rabbit pAb (AC027)

Immunofluorescence analysis of C6 cells using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC027)}

Immunofluorescence - GAPDH Rabbit pAb (AC027)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC027)}

Immunofluorescence - GAPDH Rabbit pAb (AC027)

Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

Secondary Antibodies (26)