Review (1)Publications (23) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
The multiplex IHC analysis on paraffin-embedded Mouse brain tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : NeuN Rabbit mAb (A19086, 1:2000) with TSA-TYR-520 (Green), GFAP Rabbit mAb (A19058, 1:500) with TSA-TYR-570 (Red), and TMEM119 Rabbit mAb (A27143, 1:600) with TSA-TYR-690 (Yellow). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
The multiplex IHC analysis on paraffin-embedded Rat brain tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : NeuN Rabbit mAb (A19086, 1:2000) with TSA-TYR-520 (Green), GFAP Rabbit mAb (A19058, 1:500) with TSA-TYR-570 (Red), and TMEM119 Rabbit mAb (A27143, 1:600) with TSA-TYR-690 (Yellow). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
Western blot analysis of various lysates using GFAP Rabbit mAb (A19058) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of various lysates using GFAP Rabbit mAb (A19058)at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HeLa
Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using GFAP Rabbit mAb (A19058) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human brain using GFAP Rabbit mAb (A19058) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using GFAP Rabbit mAb (A19058) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Confocal imaging of paraffin-embedded Human brain tissue using GFAP Rabbit mAb (A19058, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Mouse brain tissue using GFAP Rabbit mAb (A19058, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat brain tissue using GFAP Rabbit mAb (A19058, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
| Product name | GFAP Rabbit mAb |
|---|---|
| Catalog No. | A19058 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0206 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GFAP (P14136). |
|---|---|
| Sequence | MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAE |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ALXDRD; GFAP |
| Calculated MW | 50kDa |
| Observed MW | 48kDa/50kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | U-251 MG, Mouse brain, Rat brain, U-251 MG |
| Cellular location | Cytoplasm. |
| Customer validation | (brain slices) IHC(Rattus norvegicus, Mus musculus, Homo sapiens, Mus musculus) IF(Homo sapiens, Mus musculus, Rattus norvegicus) WB(Mus musculus , Rattus norvegicus) IF(Rattus norvegicus) IP(Mus musculus) WB(Mus musculus) ELISA(Homo sapiens) |
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