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GSDMD (Full length+N terminal) Rabbit pAb (A18281)

Publications (48) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GSDMD (Full length+N terminal) Rabbit pAb (A18281)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GSDMD (Full length+N terminal) using GSDMD (Full length+N terminal) Rabbit pAb (A18281) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 1s.

Overview

Product nameGSDMD (Full length+N terminal) Rabbit pAb
Catalog No.A18281
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human GSDMD (NP_079012.3).
SequenceDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
Gene ID
Swiss Prot
SynonymsDF5L; DFNA5L; FKSG10; GSDMDC1; GSDMD
Calculated MW53kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:400 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T
Cellular locationcytosol, extracellular region, extracellular space, NLRP3 inflammasome complex, nucleoplasm, plasma membrane.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Mus musculus, Rattus norvegicus, Gallus gallus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens, Mus musculus, Rattus norvegicus)

WB(Mus musculus, Homo sapiens, Gallus gallus)

RT-qPCR(Homo sapiens)

WB (Mus musculus)

PCR(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - GSDMD (Full length+N terminal) Rabbit pAb (A18281)}

Western blot - GSDMD (Full length+N terminal) Rabbit pAb (A18281)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GSDMD (Full length+N terminal) using GSDMD (Full length+N terminal) Rabbit pAb (A18281) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18281? Please let us know so that we can cite the reference in this datasheet.

항체 (5)

ELISA 키트 (1)

Secondary Antibodies (25)