제품 > 항체 > 다중클론항체(pAb)

GSK3β Rabbit pAb (A6164)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using GSK3β Rabbit pAb (A6164) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunoprecipitation - GSK3β Rabbit pAb (A6164)

Immunoprecipitation analysis of 200 μg extracts of 22Rv1 cells, using 3 μg GSK3β antibody (A6164). Western blot was performed from the immunoprecipitate using GSK3β antibody (A6164) at a dilution of 1:1000.

Overview

Product name	GSK3β Rabbit pAb
Catalog No.	A6164
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 320-420 of human GSK3β (NP_001139628.1).
Sequence	LLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST
Gene ID
Swiss Prot
Synonyms	GSK3B; gsk-3β; GSK3β
Calculated MW	47kDa
Observed MW	46kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	LO2, HeLa, 22Rv1, U-251MG
Cellular location	Cell membrane, Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus, Mus musculus) IF(Mus musculus) IF(Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

GSK3β Rabbit pAb images

Western blot - GSK3β Rabbit pAb (A6164)

Immunoprecipitation - GSK3β Rabbit pAb (A6164)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Neuropeptides

Application:

IF:

3.286

Species:

Homo sapiens

PMID:

32241604

Title:

Effect of (m)RVD-hemopressin against Aβ1-42-induced apoptosis and inhibition of neurite outgrowth in SH-SY5Y cells

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Diabetes Metab Syndr Obes

Application:

IF:

3.168

Species:

Homo sapiens

PMID:

32943896

Title:

Liraglutide and Insulin Have Contrary Effects on Adipogenesis of Human Adipose-Derived Stem Cells via Wnt Pathway

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Brain Research Bulletin

Application:

IF:

4.077

Species:

Mus musculus

PMID:

32272155

Title:

Morin exerts protective effects on encephalopathy and sepsis-associated cognitive functions in a murine sepsis model

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Evid Based Complement Alternat Med

Application:

IF:

2.629

Species:

Homo sapiens

PMID:

33014112

Title:

Role of Chrysophanol in Epithelial-Mesenchymal Transition in Oral Cancer Cell Lines via a Wnt-3-Dependent Pathway

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Cancer Lett

Application:

IF:

8.679

Species:

Homo sapiens

PMID:

33129955

Title:

A novel kinase inhibitor, LZT-106, downregulates Mcl-1 and sensitizes colorectal cancer cells to BH3 mimetic ABT-199 by targeting CDK9 and GSK-3β signaling

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Biochem Biophys Res Commun

Application:

IF:

3.575

Species:

Mus musculus

PMID:

33190828

Title:

Investigation for GSK3β expression in diabetic osteoporosis and negative osteogenic effects of GSK3β on bone marrow mesenchymal stem cells under a high glucose …

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Food Chem Toxicol

Application:

IF:

6.02

Species:

Mus musculus

PMID:

34411643

Title:

Inhibition of sEH via stabilizing the level of EETs alleviated Alzheimer's disease through GSK3β signaling pathway

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Acta Histochem

Application:

IF:

2.479

Species:

Mus musculus

PMID:

34217047

Title:

Calycosin attenuates pulmonary fibrosis by the epithelial-mesenchymal transition repression upon inhibiting the AKT/GSK3β/β-catenin signaling pathway

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Eur J Pharmacol

Application:

IF:

4.432

Species:

Mus musculus

PMID:

34481876

Title:

Loureirin B attenuates insulin resistance in HepG2 cells by regulating gluconeogenesis signaling pathway

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Rattus norvegicus

PMID:

34858186

Title:

Chronic Circadian Rhythm Disturbance Accelerates Knee Cartilage Degeneration in Rats Accompanied by the Activation of the Canonical Wnt/β-Catenin Signaling Pathway

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Journal of gastrointestinal oncology

Application:

IF:

2.58

Species:

Homo sapiens

PMID:

35837205

Title:

Knockdown of Obg-like ATPase 1 enhances sorafenib sensitivity by inhibition of GSK-3β/β-catenin signaling in hepatocellular carcinoma cells - PMC

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Mus musculus

PMID:

36460667

Title:

Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration - PMC

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Life Sci

Application:

IF:

6.78

Species:

Homo sapiens, Mus musculus

PMID:

32918978

Title:

Oroxylin A reversed Fibronectin-induced glioma insensitivity to Temozolomide by suppressing IP3R1/AKT/-catenin pathway

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Rattus norvegicus

PMID:

37856220

Title:

Oleanolic acid, a small-molecule natural product, inhibits ECM degeneration in osteoarthritis by regulating the Hippo/YAP and Wnt/-catenin pathways

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Microorganisms

Application:

IF:

4.5

Species:

Mus musculus

PMID:

38399751

Title:

Syringaldehyde Exhibits Antibacterial and Antioxidant Activities against Mycobacterium marinum Infection - PMC

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

PloS one

Application:

WB, IF

IF:

3.75

Species:

Mus musculus

PMID:

37058473

Title:

GSK-3 inhibition alleviates arthritis pain via reducing spinal mitochondrial reactive oxygen species level and inflammation - PMC

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Biochemical And Biophysical Research Communications

Application:

WB, IF

IF:

3.322

Species:

Rattus norvegicus

PMID:

28365156

Title:

Mild hypothermia protects against oxygen glucose deprivation/reoxygenation-induced apoptosis via the Wnt/-catenin signaling pathway in hippocampal neurons

References for GSK3β Rabbit pAb (A6164)

Product:

GSK3β Rabbit pAb

Journal:

Journal of cellular and molecular medicine

Application:

WB, IF

IF:

5.29

Species:

Rattus norvegicus

PMID:

35689386

Title:

Glycogen synthase kinase-3 inhibition decreases inflammation and relieves cancer induced bone pain via reducing Drp1-mediated mitochondrial damage - PMC