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Glucocorticoid Receptor Rabbit pAb (A2164)

Publications (14) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Glucocorticoid Receptor Rabbit pAb (A2164)

Western blot analysis of lysates from HeLa cells, using Glucocorticoid Receptor Rabbit pAb (A2164) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Glucocorticoid Receptor Rabbit pAb (A2164)

Western blot analysis of lysates from Mouse skeletal muscle, using Glucocorticoid Receptor Rabbit pAb (A2164) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunofluorescence analysis of NIH/3T3 cells using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunofluorescence analysis of PC-12 cells using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGlucocorticoid Receptor Rabbit pAb
Catalog No.A2164
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Glucocorticoid Receptor (NP_001191194.1).
SequenceMDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEK
Gene ID
Swiss Prot
SynonymsGR; GCR; GRL; GCCR; GCRST; Glucocorticoid Receptor
Calculated MW86kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2
Cellular locationCytoplasm, Mitochondrion, Nucleus, Nucleus.
Customer validation

WB(Mus musculus, Rattus norvegicus)

ChIP(Mus musculus)

IF(Rattus norvegicus)

RT-qPCR(Rattus norvegicus)

IF(Mus musculus)

ChIP(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Glucocorticoid Receptor Rabbit pAb (A2164)}

Western blot - Glucocorticoid Receptor Rabbit pAb (A2164)

Western blot analysis of lysates from HeLa cells, using Glucocorticoid Receptor Rabbit pAb (A2164) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Glucocorticoid Receptor Rabbit pAb (A2164)}

Western blot - Glucocorticoid Receptor Rabbit pAb (A2164)

Western blot analysis of lysates from Mouse skeletal muscle, using Glucocorticoid Receptor Rabbit pAb (A2164) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)}

Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)}

Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)}

Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)}

Immunohistochemistry - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Glucocorticoid Receptor Rabbit pAb (A2164)}

Immunofluorescence - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunofluorescence analysis of NIH/3T3 cells using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Glucocorticoid Receptor Rabbit pAb (A2164)}

Immunofluorescence - Glucocorticoid Receptor Rabbit pAb (A2164)

Immunofluorescence analysis of PC-12 cells using Glucocorticoid Receptor Rabbit pAb (A2164) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)