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HA-Tag Rabbit mAb (AE105)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - HA-Tag Rabbit mAb (AE105)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NFAT-HA-Tag using HA-Tag Rabbit mAb (AE105) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 10s.

ABclonal:Western blot - HA-Tag Rabbit mAb (AE105)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with LDHA-HA-Tag using HA-Tag Rabbit mAb (AE105) at 1:40000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 10s.

ABclonal:Immunofluorescence - HA-Tag Rabbit mAb (AE105)

Immunofluorescence analysis of 293T-SCRNI-HA-GFP(C) and 293T cells using HA-Tag Rabbit mAb (AE105) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HA-Tag Rabbit mAb (AE105)

Immunofluorescence analysis of 293T-SCRNI-HA-GFP(N) and 293T cells using HA-Tag Rabbit mAb (AE105) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - HA-Tag Rabbit mAb (AE105)

Immunoprecipitation analysis of 300 μg extracts of 293T-SCRN1-HA-GFP-N cells using 3 μg HA-Tag Rabbit mAb (AE105). Western blot was performed from the immunoprecipitate using HA-Tag Rabbit mAb (AE105) at a dilition of 1:6000.

Overview

Product nameHA-Tag Rabbit mAb
Catalog No.AE105
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.ARC59578
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to HA tag.
SequenceYPYDVPDYA
Gene ID
Swiss Prot
SynonymsHA; HA tag; HA-tag; HA-Tag
Calculated MW
Observed MW65kDa/40kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:60000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samples293F cells transfected with NFAT-HA-Tag, 293F cells transfected with LDHA-HA-Tag
Cellular location
Customer validation

WB(Homo sapiens, Solanum tuberosum , Mus musculus)

Co-IP(Homo sapiens)

WB(Homo sapiens, Oryctolagus cuniculus)

IF(Sus scrofa)

IP(Oryctolagus cuniculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HA-Tag Rabbit mAb (AE105)}

    Western blot - HA-Tag Rabbit mAb (AE105)

    Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NFAT-HA-Tag using HA-Tag Rabbit mAb (AE105) at 1:10000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 20 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)
    .Exposure time: 10s.
    ABclonal:Western blot - HA-Tag Rabbit mAb (AE105)}

    Western blot - HA-Tag Rabbit mAb (AE105)

    Western blot analysis of lysates from wild type (WT) and 293F cells transfected with LDHA-HA-Tag using HA-Tag Rabbit mAb (AE105) at 1:40000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 20 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)
    .Exposure time: 10s.
    ABclonal:Immunofluorescence - HA-Tag Rabbit mAb (AE105)}

    Immunofluorescence - HA-Tag Rabbit mAb (AE105)

    Immunofluorescence analysis of 293T-SCRNI-HA-GFP(C) and 293T cells using HA-Tag Rabbit mAb (AE105) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - HA-Tag Rabbit mAb (AE105)}

    Immunofluorescence - HA-Tag Rabbit mAb (AE105)

    Immunofluorescence analysis of 293T-SCRNI-HA-GFP(N) and 293T cells using HA-Tag Rabbit mAb (AE105) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - HA-Tag Rabbit mAb (AE105)}

    Immunoprecipitation - HA-Tag Rabbit mAb (AE105)

    Immunoprecipitation analysis of 300 μg extracts of 293T-SCRN1-HA-GFP-N cells using 3 μg HA-Tag Rabbit mAb (AE105). Western blot was performed from the immunoprecipitate using HA-Tag Rabbit mAb (AE105) at a dilition of 1:6000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AE105? Please let us know so that we can cite the reference in this datasheet.