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HCoV-OC43 Nucleoprotein Rabbit pAb (A20610)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:HCoV-OC43

ABclonal:Western blot - HCoV-OC43 Nucleoprotein Rabbit pAb (A20610)

Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Nucleocapsid Protein (His Tag), using HCoV-OC43 Nucleoprotein Rabbit pAb (A20610) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameHCoV-OC43 Nucleoprotein Rabbit pAb
Catalog No.A20610
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of coronavirus Nucleoprotein (YP_009555245.1).
SequenceYWYRHNRRSFKTADGNQRQLLPRWYFYYLGTGPHAKDQYGTDIDGVYWVASNQADVNTPADIVDRDPSSDEAIPTRFPPGTVLPQGYYIEGSGRSAPNSRS
Gene ID
Swiss Prot
Synonyms
Calculated MW49kDa
Observed MW50kDa
ReactivityHCoV-OC43
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman coronavirus (HCoV-OC43) Nucleocapsid Protein (His Tag)
Cellular location
Customer validation

IHC(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HCoV-OC43 Nucleoprotein Rabbit pAb (A20610)}

    Western blot - HCoV-OC43 Nucleoprotein Rabbit pAb (A20610)

    Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Nucleocapsid Protein (His Tag), using HCoV-OC43 Nucleoprotein Rabbit pAb (A20610) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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