Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using HEY1 Rabbit pAb (A16110) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
| Product name | HEY1 Rabbit pAb |
|---|---|
| Catalog No. | A16110 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 114-129 of human HEY1 (NP_001269780.1). |
|---|---|
| Sequence | AGTTASPTEPHHQGRL |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CHF2; OAF1; HERP2; HESR1; HRT-1; NERP2; hHRT1; BHLHb31; HEY1 |
| Calculated MW | 33kDa |
| Observed MW | 33kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | SH-SY5Y, Mouse brain |
| Cellular location | Nucleus. |
| Customer validation | WB(Homo sapiens, Mus musculus) IF(Mus musculus, Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A16110? Please let us know so that we can cite the reference in this datasheet.
