제품 > 항체 > 다중클론항체(pAb)

HMGB1 Rabbit pAb (A2553)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293T cells using HMGB1 Rabbit pAb (A2553) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Western blot analysis of lysates from Mouse brain using HMGB1 Rabbit pAb (A2553) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of various lysates using HMGB1 Rabbit pAb (A2553) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - HMGB1 Rabbit pAb (A2553)

Immunohistochemistry analysis of paraffin-embedded Rat brain using HMGB1 Rabbit pAb (A2553) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HMGB1 Rabbit pAb (A2553) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse heart using HMGB1 Rabbit pAb (A2553) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] HMGB1 Rabbit pAb (A2553) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] HMGB1 Rabbit pAb (A2553) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Review

Overview

Product name	HMGB1 Rabbit pAb
Catalog No.	A2553
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 69-168 of human HMGB1 (NP_002119.1).
Sequence	ARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKP
Gene ID
Swiss Prot
Synonyms	HMG1; HMG3; HMG-1; SBP-1; HMGB1
Calculated MW	25kDa
Observed MW	29kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293T, Mouse brain, MCF7, Mouse lung, Rat lung
Cellular location	Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted.
Customer validation	(Mouse brain, HeLa, Mouse spinal cord) WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Mus musculus) IHC(Rattus norvegicus, Mus musculus) IF(Mus musculus, Homo sapiens, Other) IP(Mus musculus) IF(Homo sapiens, Mus musculus) IHC(Homo sapiens, Mus musculus, Rattus norvegicus) qPCR(Mus musculus) Co-IP(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

HMGB1 Rabbit pAb images

Western blot - HMGB1 Rabbit pAb (A2553)

Immunohistochemistry - HMGB1 Rabbit pAb (A2553)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2553

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Reviews List

Product Protocols

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Allergy

Application:

IF:

12.4

Species:

Homo sapiens

PMID:

27624805

Title:

Blockade of β-catenin signaling attenuates toluene diisocyanate-induced experimental asthma

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

7.1

Species:

Mus musculus

PMID:

28845299

Title:

Enriched housing promotes post-stroke functional recovery through astrocytic HMGB1-IL-6-mediated angiogenesis

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

American journal of physiology. Renal physiology

Application:

IF:

3.7

Species:

Mus musculus

PMID:

30379096

Title:

SIRT1-mediated HMGB1 deacetylation suppresses sepsis-associated acute kidney injury.

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

American journal of physiology. Cell physiology

Application:

IHC

IF:

Species:

Rattus norvegicus

PMID:

30517029

Title:

HMGB1 is mechanistically essential in the development of experimental pulmonary hypertension.

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

American Journal of Physiology - Renal Physiology

Application:

IF:

3.7

Species:

Mus musculus

PMID:

31482731

Title:

Involvement of high-mobility group box 1 in the pathogenesis of severe 1 hemolytic uremic syndrome in a murine model

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

American Journal of Translational Research

Application:

IF:

1.7

Species:

Mus musculus

PMID:

27829992

Title:

HMGB1-TLR4 signaling participates in renal ischemia reperfusion injury and could be attenuated by dexamethasone-mediated inhibition of the ERK/NF-κB pathway.

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

3 Biotech

Application:

IHC

IF:

2.6

Species:

Mus musculus

PMID:

32818131

Title:

Lycorine attenuates lipopolysaccharide-induced acute lung injury through the HMGB1/TLRs/NF-κB pathway

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Int Immunopharmacol

Application:

IF:

4.8

Species:

Mus musculus

PMID:

33548580

Title:

The receptor for advanced glycation end products mediates dysfunction of airway epithelial barrier in a lipopolysaccharides-induced murine acute lung injury model

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Toxicol Appl Pharmacol

Application:

IF:

3.3

Species:

Mus musculus

PMID:

34755645

Title:

Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Ann Hepatol

Application:

IF:

3.7

Species:

Mus musculus

PMID:

34653689

Title:

IGF-1 alleviates CCL4-induced hepatic cirrhosis and dysfunction of intestinal barrier through inhibition TLR4/NF-κB signaling mediated by down-regulation HMGB1

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

6.9

Species:

Rattus norvegicus

PMID:

35486975

Title:

Dexmedetomidine attenuates perioperative neurocognitive disorders by suppressing hippocampal neuroinflammation and HMGB1/RAGE/NF-κB signaling pathway

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

Species:

Homo sapiens

PMID:

36718277

Title:

The von Hippel-Lindau Tumor Suppressor Gene Mutations Modulate Lipocalin-2 Expression in Ferroptotic-Inflammatory Pathways - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

The Science of the total environment

Application:

IF:

8.2

Species:

Mus musculus

PMID:

35597360

Title:

Oxidative stress mediated by the TLR4/NOX2 signalling axis is involved in polystyrene microplastic-induced uterine fibrosis in mice

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Brain sciences

Application:

IF:

3.3

Species:

Rattus norvegicus

PMID:

36552192

Title:

Electroacupuncture Alleviates Neuroinflammation by Inhibiting the HMGB1 Signaling Pathway in Rats with Sepsis-Associated Encephalopathy - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Drug delivery

Application:

IF:

6.5

Species:

Homo sapiens

PMID:

36919676

Title:

Enhanced therapeutic efficacy of doxorubicin against multidrug-resistant breast cancer with reduced cardiotoxicity - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Cellular signalling

Application:

IF:

4.4

Species:

Mus musculus

PMID:

37075875

Title:

Programmed cell death 10 increased blood-brain barrier permeability through HMGB1/TLR4 mediated downregulation of endothelial ZO-1 in glioblastoma

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

7.5

Species:

Homo sapiens

PMID:

37386564

Title:

Carbonic anhydrase IX inhibitor S4 triggers release of DAMPs related to immunogenic cell death in glioma cells via endoplasmic reticulum stress pathway - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Infection and drug resistance

Application:

IF:

2.9

Species:

Sus scrofa

PMID:

37404255

Title:

18-Glycyrrhetinic Acid Alleviates P. multocida-Induced Vascular Endothelial Inflammation by PARP1-Mediated NF-B and HMGB1 Signalling Suppression in PIEC Cells - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Cytogenetic and genome research

Application:

IF:

3.3

Species:

Other

PMID:

31268321

Title:

Prenylated Bibenzyls from the Chinese Liverwort Radula constricta and Their Mitochondria-Derived Paraptotic Cytotoxic Activities.

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

FASEB J

Application:

WB, IF

IF:

4.8

Species:

Homo sapiens

PMID:

34818449

Title:

The LPS induced pyroptosis exacerbates BMPR2 signaling deficiency to potentiate SLEPAH

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Food and chemical toxicology

Application:

WB, IF

IF:

3.9

Species:

Homo sapiens

PMID:

30578841

Title:

Corynoxine B ameliorates HMGB1-dependent autophagy dysfunction during manganese exposure in SH-SY5Y human neuroblastoma cells.

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Cellular signalling

Application:

IF:

4.4

Species:

Homo sapiens

PMID:

37543097

Title:

RNA helicase DHX33 regulates HMGB family genes in human cancer cells

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Pharmacological research

Application:

WB, IHC, IF

IF:

9.1

Species:

Homo sapiens, Mus musculus

PMID:

36746359

Title:

Liraglutide abrogates nephrotoxic effects of chemotherapies

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Journal of inflammation research

Application:

IF:

4.5

Species:

Mus musculus

PMID:

38434585

Title:

Maresin-1 Attenuates Sepsis-Associated Acute Kidney Injury via Suppressing Inflammation, Endoplasmic Reticulum Stress and Pyroptosis by Activating the AMPK/SIRT3 Pathway - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

IF:

7.7

Species:

Homo sapiens

PMID:

38331059

Title:

YAP induces FAK phosphorylation to inhibit gastric cancer cell proliferation via upregulation of HMGB1

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.7

Species:

Mus musculus

PMID:

38284775

Title:

The Ameliorative Role of Lico A on Aflatoxin B1-Triggered Hepatotoxicity Partially by Activating Nrf2 Signal Pathway

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.7

Species:

Mus musculus

PMID:

38284775

Title:

The Ameliorative Role of Lico A on Aflatoxin B1-Triggered Hepatotoxicity Partially by Activating Nrf2 Signal Pathway

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Acta Biomaterialia

Application:

IF:

9.4

Species:

Mus musculus

PMID:

31843715

Title:

A self-assembling peptide hydrogel-based drug co-delivery platform to improve tissue repair after ischemia-reperfusion injury

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Acta biochimica et biophysica Sinica

Application:

WB, IHC

IF:

3.3

Species:

Rattus norvegicus

PMID:

37222533

Title:

Rosuvastatin suppresses TNF--induced matrix catabolism, pyroptosis and senescence via the HMGB1/NF-B signaling pathway in nucleus pulposus cells - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Brain research bulletin

Application:

WB, IHC

IF:

3.5

Species:

Mus musculus

PMID:

29477834

Title:

Enriched housing promotes post-stroke neurogenesis through calpain 1-STAT3/HIF-1/VEGF signaling

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Biomolecules

Application:

IF:

5.5

Species:

Mus musculus

PMID:

35883482

Title:

Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

WB, IHC

IF:

6.2

Species:

Mus musculus

PMID:

36442400

Title:

Protective effect and mechanism of baicalin on lung inflammatory injury in BALB/cJ mice induced by PM2. 5

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.6

Species:

Mus musculus

PMID:

36144494

Title:

Recombinant Human Annexin A5 Alleviated Traumatic-Brain-Injury Induced Intestinal Injury by Regulating the Nrf2/HO-1/HMGB1 Pathway - PMC

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

Toxicology and Applied pharmacology

Application:

IHC, IF

IF:

3.3

Species:

Mus musculus

PMID:

29751049

Title:

Increasedbutyrateprimingin thegutstallsmicrobiomeassociated-gastrointestinalinflammationandhepaticmetabolicreprogrammingin amousemodelofGulfWarIllness.

References for HMGB1 Rabbit pAb (A2553)

Product:

HMGB1 Rabbit pAb

Journal:

International immunopharmacology

Application:

WB, qPCR

IF:

4.8

Species:

Mus musculus

PMID: