제품 > 항체 > 다중클론항체(pAb)

HOXB1 Rabbit pAb (A6619)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HOXB1 Rabbit pAb (A6619)

Western blot analysis of Mouse heart, using HOXB1 antibody (A6619) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - HOXB1 Rabbit pAb (A6619)

Immunofluorescence analysis of U-2 OS cells using HOXB1 antibody (A6619) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameHOXB1 Rabbit pAb
Catalog No.A6619
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXB genes located in a cluster on chromosome 17.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 35-180 of human HOXB1 (NP_002135.2).
SequenceSAQAVDSYASEGRYGGGLSSPAFQQNSGYPAQQPPSTLGVPFPSSAPSGYAPAACSPSYGPSQYYPLGQSEGDGGYFHPSSYGAQLGGLSDGYGAGGAGPGPYPPQHPPYGNEQTASFAPAYADLLSEDKETPCPSEPNTPTARTF
Gene ID
Swiss Prot
SynonymsHOX2; HCFP3; HOX2I; Hox-2.9
Calculated MW32kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:4000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse heart
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - HOXB1 Rabbit pAb (A6619)}

Western blot - HOXB1 Rabbit pAb (A6619)

Western blot analysis of Mouse heart, using HOXB1 antibody (A6619) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - HOXB1 Rabbit pAb (A6619)}

Immunofluorescence - HOXB1 Rabbit pAb (A6619)

Immunofluorescence analysis of U-2 OS cells using HOXB1 antibody (A6619) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6619? Please let us know so that we can cite the reference in this datasheet.