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HP1 alpha/CBX5 Rabbit mAb (A3741)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HP1 alpha/CBX5 Rabbit mAb (A3741)

Western blot analysis of various lysates using HP1 alpha/CBX5Rabbit mAb (A3741) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - HP1 alpha/CBX5 Rabbit mAb (A3741)

Western blot analysis of various lysates using HP1 alpha/CBX5Rabbit mAb (A3741) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - HP1 alpha/CBX5 Rabbit mAb (A3741)

Confocal imaging of C6 cells using HP1 alpha/CBX5 Rabbit mAb (A3741, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - HP1 alpha/CBX5 Rabbit mAb (A3741)

Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg HP1 alpha/CBX5 antibody (A3741). Western blot was performed from the immunoprecipitate using HP1 alpha/CBX5 antibody (A3741) at a dilution of 1:1000.

Overview

Product nameHP1 alpha/CBX5 Rabbit mAb
Catalog No.A3741
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0244
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 92-191 of human HP1 alpha/CBX5 (P45973).
SequenceSNFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKETAKS
Gene ID
Swiss Prot
SynonymsHP1; HP1A; HEL25; HP1 alpha/CBX5
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:200 - 1:800
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, MCF7, Mouse lung, Mouse brain, Rat lung, Rat brain
Cellular locationChromosome, Nucleus, centromere.
Customer validation

ChIP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Western blot - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Western blot analysis of various lysates using HP1 alpha/CBX5Rabbit mAb (A3741) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Western blot - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Western blot analysis of various lysates using HP1 alpha/CBX5Rabbit mAb (A3741) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunofluorescence - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Confocal imaging of C6 cells using HP1 alpha/CBX5 Rabbit mAb (A3741, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunoprecipitation - HP1 alpha/CBX5 Rabbit mAb (A3741)}

    Immunoprecipitation - HP1 alpha/CBX5 Rabbit mAb (A3741)

    Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg HP1 alpha/CBX5 antibody (A3741). Western blot was performed from the immunoprecipitate using HP1 alpha/CBX5 antibody (A3741) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A3741? Please let us know so that we can cite the reference in this datasheet.

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