Western blot - HP1 alpha/CBX5 Rabbit mAb (A3741)
Western blot analysis of various lysates using HP1 alpha/CBX5Rabbit mAb (A3741) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot - HP1 alpha/CBX5 Rabbit mAb (A3741)
Western blot analysis of various lysates using HP1 alpha/CBX5Rabbit mAb (A3741) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using HP1 alpha/CBX5 Rabbit mAb (A3741) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence - HP1 alpha/CBX5 Rabbit mAb (A3741)
Confocal imaging of C6 cells using HP1 alpha/CBX5 Rabbit mAb (A3741, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation - HP1 alpha/CBX5 Rabbit mAb (A3741)
Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg HP1 alpha/CBX5 antibody (A3741). Western blot was performed from the immunoprecipitate using HP1 alpha/CBX5 antibody (A3741) at a dilution of 1:1000.