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HRP-conjugated β-Actin Rabbit mAb (AC028)

Publications (64) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

ABclonal:Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)

Western blot analysis of various lysates using HRP-conjugated β-Actin Rabbit mAb (AC028) at 1:50000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)

Western blot analysis of lysates from HeLa, using HRP-conjugated β-Actin Rabbit mAb (AC028) at 1:1000- 1:512000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameHRP-conjugated β-Actin Rabbit mAb
Catalog No.AC028
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5115-01
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
ImmunogenRecombinant protein of human HRP-conjugated β-Actin
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsBRWS1; PS1TP5BP1; HRP-conjugated β-Actin
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:50000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F, HeLa, HepG2, NIH/3T3, Mouse brain, C6
Cellular locationCytoplasm, cytoskeleton.
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa, Gallus gallus, Aves, Oncorhynchus mykiss, Mus musculus、Sus scrofa, Mus musculus, Zea mays, Drosophila melanogaster, Other, Ctenopharyngodon idellus)

Other(Bos taurus, Mus musculus)

Documents

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ABclonal:Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)}

Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)

Western blot analysis of various lysates using HRP-conjugated β-Actin Rabbit mAb (AC028) at 1:50000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)}

Western blot - HRP-conjugated β-Actin Rabbit mAb (AC028)

Western blot analysis of lysates from HeLa, using HRP-conjugated β-Actin Rabbit mAb (AC028) at 1:1000- 1:512000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

재조합 단백질 (1)

ELISA 키트 (1)

Secondary Antibodies (26)