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HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Publications (1686) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rabbit

ABclonal:HRP-conjugated Goat anti-Rabbit IgG (H+L)
ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells, using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of paraffin-embedded Human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameHRP-conjugated Goat anti-Rabbit IgG (H+L)
Catalog No.AS014
Host speciesGoat
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
ReactivityRabbit
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • DB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
  • ELISA 1:5000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples
Cellular location
Customer validation

WB(Drosophila, Homo sapiens, Mus musculus, Rattus norvegicus, Brachyura, Oryza sativa, Crassostrea gigas, Saccharomyces cerevisiae, Caenorhabditis Elegans, Sus scrofa, Gallus gallus, Oyster, Cavia porcellus, Squaliobarbus curriculus, Oryctolagus cuniculus, M. truncatula, Malus micromalus, Tegillarca granosa, Zea mays, Drosophila melanogaster, Riemerella anatipestifer, Spodoptera frugiperda, Giardia duodenalis, Ictalurus punctatus, Eospalax cansus, Triticum aestivum, Solanum tuberosum, Cryptosporidium parvum, Ctenopharyngodon idellus, Toxoplasma gondii, Danio rerio, Cyprinus carpio, Escherichia coli, Plutella xylostella, Haliotis, Mesocricetus auratus, Carassius auratus gibelio, Bombyx mori Linnaeus, Chlorocebus aethiops, Gossypium spp, Mus musculus、Sus scrofa, Capra hircus, Arabidopsis thaliana, Ostrinia furnacalis , Ovis aries, Sus domesticus, Ctenopharyngodon idella, A. fumigatus, Pseudosciaena crocea, Pyropia yezoensis, Zea mays L. , Actinopterygii, Staphylococcus aureus, Ark clam, Nicotiana tabacum, Bos taurus, Pampus argenteus, Monkeypox virus, Tomentosa, C. elegans, Yellow catfish, Rattus norvegicus, Mytilus coruscus , Mus musculus, S. cerealella, A. fumigatus strains, Anser cygnoides, Homo sapiens , Chlorocebus aethiops, Cabbage beetles, Other, Macaca fascicularis, Monkey, Populus trichocarpa Torr. & Gray, Canis familiaris, Bos taurus, Danio rerio, Misgurnus anguillicaudatus, Xenopus laevis, Felis catus)

IHC(Homo sapiens, Scophthalmus maximus, Mus musculus, Rattus norvegicus, Cervus nippon, Oryctolagus cuniculus, Bos taurus, Other, Toxoplasma gondii)

(Mus musculus)

(Mus musculus)

IP(Mus musculus)

IF(Mus musculus, Felis catus, Homo sapiens, Rattus norvegicus, Oryctolagus cuniculus, Mus musculus, Arabidopsis thaliana)

IF(Homo sapiens, Capra hircus, Mus musculus, Rattus norvegicus, Gallus gallus, Rattus norvegicus)

IHC(Rattus norvegicus, Mus musculus, Homo sapiens, Mus musculus, Gallus gallus, Misgurnus anguillicaudatus)

ELISA(Carassius auratus gibelio, Mus musculus, Rattus norvegicus, Crassostrea gigas, Homo sapiens)

FC(Homo sapiens, Other)

ELISA(Sus scrofa, SARS-CoV-2, Homo sapiens, Ctenopharyngodon idellus, Oryctolagus cuniculus, Mus musculus, Rattus norvegicus)

DB(Homo sapiens, Mus musculus)

WB(Homo sapiens)

RT-PCR(Mus musculus, Homo sapiens, Mus musculus)

IP(Homo sapiens )

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa, Arabidopsis thaliana, Other)

IHC(Rattus norvegicus, Mus musculus, Homo sapiens)

Other(OystersC. gigas, Mus musculus, Homo sapiens, Other)

DB(Homo sapiens)

FC(Homo sapiens)

Co-IP(Homo sapiens, Mus musculus, Xenopus laevis)

Neutralization(Other)

Co-IP(Homo sapiens, Other)

ChIP(Mus musculus, Homo sapiens)

IF(Rattus norvegicus, Homo sapiens)

Co-IP(Homo sapiens)

FC(Homo sapiens)

RT-qPCR(Homo sapiens)

qPCR(Other)

RT-qPCR(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

    Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

    Western blot analysis of lysates from HeLa cells, using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
    Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

    Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

    Immunohistochemistry analysis of paraffin-embedded Human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS014? Please let us know so that we can cite the reference in this datasheet.