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HRP-conjugated Goat anti-Rat IgG (H+L) (AS028)

Publications (22) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:HRP-conjugated Goat anti-Rat IgG (H+L)
ABclonal:Western blot - HRP-conjugated Goat anti-Rat IgG (H+L) (AS028)

Western blot analysis of lysates from Rat IgG, using HRP Goat Anti-Rat IgG (H+L) (AS028) at 1:1000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - HRP-conjugated Goat anti-Rat IgG (H+L) (AS028)

Western blot analysis of lysates from Rat IgG using HRP Goat Anti-Rat IgG (H+L) (AS028) at 1:5000 dilution.
Lysates/proteins: 50ng - 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameHRP-conjugated Goat anti-Rat IgG (H+L)
Catalog No.AS028
Host speciesGoat
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRat IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IHC-P 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRabbit IgG, Mouse IgG, Rat IgG
Cellular location
Customer validation

WB(Rattus norvegicus, Mus musculus, granulosus, Eriocheir sinensis, Homo sapiens, Mus musculus, Other, Toxoplasma gondii)

IHC(Actinopterygii, Mus musculus)

IF(Rattus norvegicus)

ELISA(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - HRP-conjugated Goat anti-Rat IgG (H+L) (AS028)}

    Western blot - HRP-conjugated Goat anti-Rat IgG (H+L) (AS028)

    Western blot analysis of lysates from Rat IgG, using HRP Goat Anti-Rat IgG (H+L) (AS028) at 1:1000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - HRP-conjugated Goat anti-Rat IgG (H+L) (AS028)}

    Western blot - HRP-conjugated Goat anti-Rat IgG (H+L) (AS028)

    Western blot analysis of lysates from Rat IgG using HRP Goat Anti-Rat IgG (H+L) (AS028) at 1:5000 dilution.
    Lysates/proteins: 50ng - 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS028? Please let us know so that we can cite the reference in this datasheet.