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HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Western blot - HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095)

Western blot analysis of extracts of normal 293T cells, 293T transfected with GSK3B-C Protein, using HRP-conjugated rabbit anti DDDDK-Tag mAb (AE095) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095)

Western blot analysis of extracts of normal 293F，293F transfected with IRF1-N Protein, using HRP-conjugated rabbit anti DDDDK-Tag mAb (AE095) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameHRP-conjugated Rabbit anti DDDDK-Tag mAb
Catalog No.AE095
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5111-01-HRP
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
ImmunogenA synthetic peptide corresponding to DDDDK tag.
SequenceDYKDDDDK
Gene ID
Swiss Prot
SynonymsDDDDK; DDDDK tag; DDDDK-tag
Calculated MW
Observed MW50kDa/46kDa
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000-6000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F, 293T
Cellular location

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Lot number

    ABclonal:Western blot - HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095)}

    Western blot - HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095)

    Western blot analysis of extracts of normal 293T cells, 293T transfected with GSK3B-C Protein, using HRP-conjugated rabbit anti DDDDK-Tag mAb (AE095) at 1:5000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095)}

    Western blot - HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095)

    Western blot analysis of extracts of normal 293F，293F transfected with IRF1-N Protein, using HRP-conjugated rabbit anti DDDDK-Tag mAb (AE095) at 1:5000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.