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HRP-conjugated β-Tubulin Mouse mAb (AC030)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using HRP-conjugated β-Tubulin Mouse mAb (AC030) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	HRP-conjugated β-Tubulin Mouse mAb
Catalog No.	AC030
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG1, Kappa
CloneNo.	AMC0499

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Immunogen information

Immunogen	Recombinant protein of human TUBB
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; HRP-conjugated β-Tubulin
Calculated MW	50kDa
Observed MW	55kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:10000 - 1:50000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, U-87MG, 293T, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain
Cellular location	Cytoplasm, cytoskeleton.
Customer validation	WB(Rattus norvegicus, Homo sapiens, Mus musculus, Drosophila melanogaster) IHC(Homo sapiens) IP(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

HRP-conjugated β-Tubulin Mouse mAb images

Western blot - HRP-conjugated β-Tubulin Mouse mAb (AC030)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

American journal of physiology. Renal physiology

Application:

IF:

4.09

Species:

Rattus norvegicus

PMID:

35466690

Title:

SGLT2 inhibition effect on salt-induced hypertension, RAAS, and Na + transport in Dahl SS rats

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Frontiers in pediatrics

Application:

IF:

3.56

Species:

Rattus norvegicus

PMID:

36969270

Title:

Dose-dependent effects of human umbilical cord-derived mesenchymal stem cell treatment in hyperoxia-induced lung injury of neonatal rats - PMC

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Computational and structural biotechnology journal

Application:

IF:

6.15

Species:

Homo sapiens

PMID:

37389187

Title:

Impact of Cuproptosis-related markers on clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: A multi-omic analysis - PMC

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

PLoS pathogens

Application:

IF:

7.46

Species:

Homo sapiens

PMID:

37948454

Title:

Antibody-mediated spike activation promotes cell-cell transmission of SARS-CoV-2 - PMC

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Cytokine

Application:

IF:

3.92

Species:

Homo sapiens

PMID:

37480791

Title:

TRAF6-TAK1-IKK pathway mediates TLR2 agonists activating "one-step" NLRP3 inflammasome in human monocytes

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

PeerJ

Application:

IHC

IF:

3.06

Species:

Homo sapiens

PMID:

37609434

Title:

Identification of hub genes associated with oxidative stress in heart failure and their correlation with immune infiltration using bioinformatics analysis - PMC

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Proc Natl Acad Sci U S A

Application:

IF:

12.779

Species:

Homo sapiens, Mus musculus

PMID:

34930824

Title:

SARS-CoV-2 spike engagement of ACE2 primes S2 site cleavage and fusion initiation

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Cell reports

Application:

IF:

Species:

Homo sapiens

PMID:

37074913

Title:

Structures of the human Wilson disease copper transporter ATP7B

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Frontiers in cellular and infection microbiology

Application:

WB, IP

IF:

6.07

Species:

Mus musculus

PMID:

36936774

Title:

BPOZ-2 is a negative regulator of the NLPR3 inflammasome contributing to SARS-CoV-2-induced hyperinflammation - PMC

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Drosophila melanogaster

PMID:

37543696

Title:

Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1 - PMC

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Nature

Application:

IF:

69.5

Species:

Homo sapiens

PMID:

37532940

Title:

GPCR activation and GRK2 assembly by a biased intracellular agonist

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Nucleic acids research

Application:

IF:

14.9

Species:

Drosophila melanogaster

PMID:

38499485

Title:

Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

iScience

Application:

IF:

6.1

Species:

Rattus norvegicus

PMID:

36039296

Title:

Lack of xanthine dehydrogenase leads to a remarkable renal decline in a novel hypouricemic rat model - PMC

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Life science alliance

Application:

IF:

4.4

Species:

Mus musculus

PMID:

38918041

Title:

Nrg1 intracellular signaling regulates the development of interhemispheric callosal axons in mice - PMC

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Drosophila melanogaster,Homo sapiens

PMID:

38837887

Title:

Minor Spliceosomal 65K/RNPC3 Interacts with ANKRD11 and Mediates HDAC3Regulated Histone Deacetylation and Transcription

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Genome research

Application:

IF:

6.2

Species:

Drosophila melanogaster

PMID:

37973194

Title:

tRNA-derived small RNAs are embedded in the gene regulatory program instructing Drosophila metamorphosis

References for HRP-conjugated β-Tubulin Mouse mAb (AC030)

Product:

HRP-conjugated β-Tubulin Mouse mAb

Journal:

Clinical science (London, England : 1979)

Application:

IF:

6.7

Species:

Rattus norvegicus

PMID:

38358003

Title:

The presence of xanthine dehydrogenase is crucial for the maturation of the rat kidneys

Publishing research using AC030? Please let us know so that we can cite the reference in this datasheet.

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제품 > 항체 > Tag & 로딩 컨트롤 항체

HRP-conjugated β-Tubulin Mouse mAb (AC030)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

HRP-conjugated β-Tubulin Mouse mAb images

Product Protocols

항체 (4)

Secondary Antibodies (22)