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HRP polymer-conjugated Goat Anti-Mouse Anti-Rabbit IgG H (AS080)

Publications (25) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - HRP polymer-conjugated Goat Anti-Mouse Anti-Rabbit IgG H (AS080)

Western blot analysis of various lysates using Goat Anti-Mouse Anti-Rabbit IgG H(HRP polymer) (AS080) at 1:1000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameHRP polymer-conjugated Goat Anti-Mouse Anti-Rabbit IgG H
Catalog No.AS080
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenA synthetic peptide corresponding to a sequence within both Mouse IgG and Rabbbit IgG.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW55kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse IgG, Rabbit IgG
Cellular location
Customer validation

WB(Homo sapiens, Chlamydomonas reinhardtii, Mus musculus, Mus musculus, Rattus norvegicus, Bos taurus, Oryctolagus cuniculus, Other)

IHC(Homo sapiens, Mus musculus)

ELISA(Mus musculus)

Other(Bos taurus)

Other(Bos taurus)

Cell transfection(Rattus norvegicus)

Documents

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    ABclonal:Western blot - HRP polymer-conjugated Goat Anti-Mouse Anti-Rabbit IgG H (AS080)}

    Western blot - HRP polymer-conjugated Goat Anti-Mouse Anti-Rabbit IgG H (AS080)

    Western blot analysis of various lysates using Goat Anti-Mouse Anti-Rabbit IgG H(HRP polymer) (AS080) at 1:1000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS080? Please let us know so that we can cite the reference in this datasheet.