Description
Heat-T4 DNA Ligase is a thermostable mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. It is designed to function at higher temperatures than wild-type T4 DNA ligase. The catalytic reaction requires ATP as a cofactor. Heat-T4 DNA Ligase will join blunt end and cohesive end termini at temperatures as high as 45ºC , and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.
Product Features
- Improves the thermal stability of the connection reaction.
- Maintain more than 90% activity at temperatures up to 45 ° C for 24 hours.
- It can catalyze the formation of phosphodilipid bond at flat end and sticky end, and repair double-stranded DNA, RNA, DNA/RNA hybrid strand cutting.
- strict batch performance and stability quality control.
Applications
Cloning of restriction fragments
Joining linkers and adapters to blunt-ended DNA
Storage
-20°C
Product Components
| Components | 20, 000 U | 100, 000 U |
| Heat-T4 DNA Ligase (400, 000 U/mL) | 50 μL | 250 μL |
| 10X T4 DNA Ligase Reaction Buffer | 500 μL | 500 μL |
Product Data
- The same amount of Heat-T4 DNA Ligase (ABclonal, RK21507) and Heat T4 DNA Ligase (rival N) from other manufacturers were used for high temperature enzyme activity tolerance test (0-24h, 45℃). The results showed that Heat-T4 DNA Ligase (ABclonal, RK21507) maintained more than 90% activity at 45 ° C for 24h, which was comparable to the competitive performance.
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