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Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Review (1)Publications (146) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Western blot analysis of lysates from A549 cells, using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Western blot analysis of lysates from NIH/3T3 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Western blot analysis of various lysates using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunohistochemistry analysis of paraffin-embedded Human liver using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunofluorescence analysis of HeLa cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunofluorescence analysis of HepG2 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb(A1346)

Overview

Product nameHeme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb
Catalog No.A1346
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-288 of human Heme Oxygenase 1 (HO-1/HMOX1) (NP_002124.1).
SequenceMERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM
Gene ID
Swiss Prot
SynonymsHO-1; HSP32; HMOX1D; bK286B10; Heme Oxygenase 1 (HO-1/HMOX1)
Calculated MW33kDa
Observed MW33kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA549, NIH/3T3, Hep G2, , HeLa
Cellular locationCytoplasmic side, Endoplasmic reticulum membrane, Microsome, Peripheral membrane protein.
Customer validation

(Mouse liver)

WB(Mus musculus, Rattus norvegicus, Struthio camelus, Homo sapiens, Cyprinus carpio, Gallus gallus, Vaccinium dunalianum, Bos taurus, Oryza sativa, Sus scrofa, Chrysanthemum, Other)

IF(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

WB(Mus musculus)

IHC(Rattus norvegicus, Chrysanthemum)

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)}

Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Western blot analysis of lysates from A549 cells, using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)}

Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Western blot analysis of lysates from NIH/3T3 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)}

Western blot - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Western blot analysis of various lysates using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)}

Immunohistochemistry - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunohistochemistry analysis of paraffin-embedded Human liver using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)}

Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunofluorescence analysis of HeLa cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)}

Immunofluorescence - Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346)

Immunofluorescence analysis of HepG2 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (A1346) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

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WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse，Rat

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WBIHC-PELISA

Reactivity:Human, Mouse, Rat

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A21911

WBELISA

Reactivity:Human

Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)