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Histone H3 Rabbit pAb (A2348)

Reviews (5)Publications (177) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Histone H3 Rabbit pAb
ABclonal:Western blot - Histone H3 Rabbit pAb (A2348)

Western blot analysis of various lysates using Histone H3 Rabbit pAb (A2348) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of paraffin-embedded Rat lung using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of paraffin-embedded Human liver using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of paraffin-embedded Human brain using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Histone H3 Rabbit pAb (A2348)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse testis using Histone H3 Rabbit pAb (A2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Histone H3 Rabbit pAb (A2348)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat testis using Histone H3 Rabbit pAb (A2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (A2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (A2348) at a dilution of 1:30000.

ABclonal:Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (A2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (A2348) at a dilution of 1:30000.

ABclonal:Chromatin Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

Chromatin immunoprecipitation analysis of extracts of 293T cells, using Histone H3 antibody (A2348) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)
Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)
Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)
Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)
Review
ABclonal: review for Histone H3 Rabbit pAb(A2348)

Overview

Product nameHistone H3 Rabbit pAb
Catalog No.A2348
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3 (NP_003520.1).
SequenceVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
Gene ID
Swiss Prot
SynonymsH3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Histone H3
Calculated MW16kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 3μg antibody for 5μg-10μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293F, NIH/3T3, PC-12
Cellular locationChromosome, Nucleus.
Customer validation

(Mouse embryonic fibroblasts, Raw264.7, Mouse E16.5 neocortex, Rat brain, HNSCs)

WB(Homo sapiens, Mus musculus, Saccharomyces cerevisiae, Rattus norvegicus, Arabidopsis thaliana, Danio rerio, Bombyx mori Linnaeus, Plasmodium falciparum, Gallus gallus, P. xylostella, Oryza sativa, Drosophila, M. japonicus, Monascus ruber, Eurosta solidaginis, Mus musculus, Rattus norvegicus, Penaeus japonicus, Glycine max, Zea mays, Ictalurus punctatus, Sus scrofa, Nicotiana benthamiana, Other)

IF(Homo sapiens, ocu, Mus musculus, Mus musculus , Arabidopsis thaliana)

IP(Helicoverpa armigera, Homo sapiens)

WB(Nicotiana benthamiana , Homo sapiens, Arabidopsis thaliana)

IHC(Homo sapiens, Mus musculus)

IP(Homo sapiens)

ChIP-Seq(Arabidopsis thaliana)

ChIP(Homo sapiens, Arabidopsis thaliana)

IF(Homo sapiens)

Other(Other, Homo sapiens)

Co-IP(Homo sapiens)

FC(Homo sapiens)

IF(Homo sapiens)

RT-qPCR(Homo sapiens)

Other(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Histone H3 Rabbit pAb (A2348)}

    Western blot - Histone H3 Rabbit pAb (A2348)

    Western blot analysis of various lysates using Histone H3 Rabbit pAb (A2348) at 1:10000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)}

    Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

    Immunohistochemistry analysis of paraffin-embedded Rat lung using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)}

    Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

    Immunohistochemistry analysis of paraffin-embedded Human liver using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)}

    Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

    Immunohistochemistry analysis of paraffin-embedded Human brain using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Histone H3 Rabbit pAb (A2348)}

    Immunohistochemistry - Histone H3 Rabbit pAb (A2348)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Histone H3 Rabbit pAb (A2348) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Histone H3 Rabbit pAb (A2348)}

    Immunofluorescence - Histone H3 Rabbit pAb (A2348)

    Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse testis using Histone H3 Rabbit pAb (A2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Histone H3 Rabbit pAb (A2348)}

    Immunofluorescence - Histone H3 Rabbit pAb (A2348)

    Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat testis using Histone H3 Rabbit pAb (A2348) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Histone H3 Rabbit pAb (A2348)}

    Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

    Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (A2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (A2348) at a dilution of 1:30000.
    ABclonal:Immunoprecipitation - Histone H3 Rabbit pAb (A2348)}

    Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

    Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 10ug Histone H3 antibody (A2348). Western blot was performed from the immunoprecipitate using Histone H3 antibody (A2348) at a dilution of 1:30000.
    ABclonal:Chromatin Immunoprecipitation - Histone H3 Rabbit pAb (A2348)}

    Chromatin Immunoprecipitation - Histone H3 Rabbit pAb (A2348)

    Chromatin immunoprecipitation analysis of extracts of 293T cells, using Histone H3 antibody (A2348) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    항체 (105)

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