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Hsp90α/β Rabbit mAb (A5027)

Reviews (2)Publications (15) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Hsp90α/β Rabbit mAb (A5027)

Western blot analysis of various lysates using Hsp90α/β Rabbit mAb (A5027) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for Hsp90α/β Rabbit mAb(A5027)
Review
ABclonal: review for Hsp90α/β Rabbit mAb(A5027)

Overview

Product nameHsp90α/β Rabbit mAb
Catalog No.A5027
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1169
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 533-732 of human Hsp90α (P07900).
SequenceLKEFEGKTLVSVTKEGLELPEDEEEKKKQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEGDDDTSRMEEVD
Gene ID
Swiss Prot
SynonymsEL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2; Hsp90α/β
Calculated MW90kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Raji, Mouse liver, Rat liver, Rat brain
Cellular locationCell membrane, Cytoplasm, Melanosome.
Customer validation

(human keratinocyte, 143B cells)

WB(Mus musculus, Homo sapiens, Gallus gallus)

IHC(Mus musculus)

IF(Mus musculus)

ELISA(Mus musculus)

Other(Other)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Hsp90α/β Rabbit mAb (A5027)}

    Western blot - Hsp90α/β Rabbit mAb (A5027)

    Western blot analysis of various lysates using Hsp90α/β Rabbit mAb (A5027) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)