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Human HER2 Monoclonal Antibody (YR0015)

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human ErbB2/HER2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody to ErbB2/HER2. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A donkey anti-human IgG HRP conjugate was used for detection.

Determined through SPR assay, the Human Anti-Human ErbB2/HER2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody is capable of binding to Human ErbB2/HER2 with an affinity constant of 0.4389 nM.

ABclonal:Flow CytoMetry - Human HER2 Monoclonal Antibody (YR0015)

Detection of ErbB2/HER2 in SK-BR-3 cell line by Flow Cytometry. SK-BR-3 cell line was stained with Human Anti-Human ErbB2/HER2 (Trastuzumab Biosimilar) Monoclonal Antibody followed by APC conjugated Anti-Human IgG Secondary Antibody, or unstained cells (open yellow histogram).

Basic Information

Clone	Trastuzumab Biosimilar
Molecular Weight	150 kDa
Endotoxin	<1EU/mg (<0.001EU/μg)Determined by LAL gel clotting assay
Sterility	0.2 μm filtration
Aggregation	<5% Determined by SECP
Purity	>95% Determined by SEC-HPLC

Product Information

Production	Purified from cell culture supernatant in an animal-free facility
Purification	Protein A or G purification
Storage	2 - 8°C for up to 4 weeks and -80°C for long term storage (Avoid repeated freezing and thawing)

Target Background

Trastuzumab, a humanized monoclonal antibody, interferes with the HER2/neu receptor and is mainly used to treat certain breast cancers, especially HER2-positive metastatic breast cancer. Combined with chemotherapy, trastuzumab increases both survival and response rate.There are four closely related receptor tyrosine kinases in the ErbB family of receptors: EGFR (ErbB-1; HER1 in humans), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Bound by members of the epidermal growth factor family (EGF-family) of extracellular protein ligands, the HER receptors embedded in the cell membrane promote cell growth and division by turning genes on and off. The HER2 pathway stimulates cell proliferation if it normally functions; when HER2 is overexpressed, cell growth accelerates beyond its normal limits, which happens in some cancers, notably certain types of breast cancer.

Immunogen Information

Isotype	Human IgG1 kappa
Immunogen	human HER2
RecommendedIsotype Control(s)	In Vivo Grade Recombinant Human IgG1 Kappa Isotype Control Antibody
Recommended Dilution Buffer	1×PBS pH 7.0