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Human PD-1 Monoclonal Antibody (YR0001)

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human PD-1 (Research Grade Pembrolizumab Biosimilar) Monoclonal Antibody to PD-1. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A donkey anti-human IgG HRP conjugate was used for detection.

Determined through SPR assay, the Human Anti-Human PD-1 (Research Grade Pembrolizumab Biosimilar) Monoclonal Antibody is capable of binding to Human PD-1 with an affinity constant of 1.282 nM.

ABclonal:Flow CytoMetry - Human PD-1 Monoclonal Antibody (YR0001)

Detection of PD-1 in HEK 293 cells by Flow Cytometry. HEK 293 cells transfected hPD-1 was stained with Human Anti-Human PD-1 (Research Grade Pembrolizumab Biosimilar) Monoclonal Antibody followed by APC conjugated Anti-Human IgG Secondary Antibody, or unstained cells (open yellow histogram).

Basic Information

Clone	Pembrolizumab Biosimilar
Molecular Weight	150 kDa
Endotoxin	<1EU/mg (<0.001EU/μg)Determined by LAL gel clotting assay
Sterility	0.2 μm filtration
Aggregation	<5% Determined by SECP
Purity	>95% Determined by SEC-HPLC

Product Information

Production	Purified from cell culture supernatant in an animal-free facility
Purification	Protein A or G purification
Storage	2 - 8°C for up to 4 weeks and -80°C for long term storage (Avoid repeated freezing and thawing)

Target Background

What is Pembrolizumab biosimilar research grade? Pembrolizumab is a highly selective IgG4-kappa humanized monoclonal antibody against PD-1 receptor. It was generated by grafting the variable sequences of a very high-affinity mouse antihuman PD-1 antibody onto a human IgG4-kappa isotype with the containing a stabilizing S228P Fc mutation. It contains 32 cysteine residues and the complete folded molecule includes 4 disulfide linkages as interchain bonds and 23 interchain bonds. Pembrolizumab Biosimilar uses the same protein sequences as the therapeutic antibody pembrolizumab.

Immunogen Information

Isotype	human IgG4 kappa
Immunogen	Human PD-1
RecommendedIsotype Control(s)	In Vivo Grade Recombinant Human IgG4-S228P Kappa Isotype Control Antibody
Recommended Dilution Buffer	1×PBS pH 7.0

Human PD-1 Monoclonal Antibody images

- Human PD-1 Monoclonal Antibody (YR0001)

Determined through SPR assay, the Human Anti-Human PD-1 (Research Grade Pembrolizumab Biosimilar) Monoclonal Antibody is capable of binding to Human PD-1 with an affinity constant of 1.282 nM.

Flow CytoMetry - Human PD-1 Monoclonal Antibody (YR0001)

* For research use only. Not for therapeutic or diagnostic purposes.