Basic Information
Clone | Envafolimab Biosimilar |
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Molecular Weight | 150 kDa |
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Endotoxin | <1EU/mg (<0.001EU/μg)Determined by LAL gel clotting assay |
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Sterility | 0.2 μm filtration |
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Aggregation | <5% Determined by SECP |
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Purity | >95% Determined by SDS-PAGE |
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Product Information
Production | Purified from cell culture supernatant in an animal-free facility |
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Purification | Protein A or G purification |
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Storage | 2 - 8°C for up to 4 weeks and -80°C for long term storage (Avoid repeated freezing and thawing) |
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Target Background
Envafolimab is a humanized nanobody (VHH-hIgG1 Fc fusion protein) directed against the human protein ligand PD-L1, with potential immune checkpoint inhibitory and antineoplastic activities. Envafolimab biosimilar uses the same protein sequences as the therapeutic antibody envafolimab.PD-L1 (B7-H1 or CD274, programmed cell death ligand 1) and PD-L2 (B2-DC or CD273, programmed cell death ligand 2) are the two ligands for the receptor PD-1 (CD279, programmed death 1). PD-L1 is an immune checkpoint molecule expressed on both tumor cells and certain immune cells. The binding of PD-L1 to its receptors PD-1 or B7.1 on activated T cells causes an inhibitory signal to suppress their production in the lymph nodes, thereby preventing immune responses to events such as pregnancy or autoimmune disease. This pathway is also utilized by cancer cells to evade the immune system through evasion of anti-tumor T-cell response. Furthermore, over-expression of PD-L1 and PD-1 has been linked to increased tumor aggressiveness and poorer prognosis. Envafolimab binds directly and selectively to PD-L1 and blocks interaction with both PD-1 and B7.1 receptors, thus reinvigorates and enhances the body’s adaptive anti-cancer activity. Disrupting the PD-L1/B7.1 interaction may also enhance T-cell priming, which could result in increased duration of response and survival.
Immunogen Information
Isotype | Human IgG1 Fc |
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Immunogen | Human CD274 |
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RecommendedIsotype Control(s) | In Vivo Grade Recombinant Human IgG1 Fc Protein |
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Recommended Dilution Buffer | 1×PBS pH 7.0 |
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* For research use only. Not for therapeutic or diagnostic purposes.