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Human TNF alpha Monoclonal Antibody (YR0013)

Datasheet

ABclonal: - Human TNF alpha Monoclonal Antibody (YR0013)

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human TNF-α (Research Grade Adalimumab Biosimilar) Monoclonal Antibody to TNF-α. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A donkey anti-human IgG HRP conjugate was used for detection.

ABclonal: - Human TNF alpha Monoclonal Antibody (YR0013)

Determined through SPR assay, the Human Anti-Human TNF-α (Research Grade Adalimumab Biosimilar) Monoclonal Antibody is Capable of binding to Human TNF-α with an affinity constant of 0.1658 M.

Basic Information

CloneAdalimumab Biosimilar
Molecular Weight150 kDa
Endotoxin<1EU/mg (<0.001EU/μg)Determined by LAL gel clotting assay
Sterility0.2 μm filtration
Aggregation<5% Determined by SECP
Purity94.03% Determined by SEC-HPLC
ProductionPurified from cell culture supernatant in an animal-free facility
PurificationProtein A or G purification
Storage2 - 8°C for up to 4 weeks and -80°C for long term storage (Avoid repeated freezing and thawing)
Adalimumab, the first fully human monoclonal antibody drug approved by FDA, binds to TNFα, inactivates TNF receptors, and downregulates the inflammatory reactions associated with a variety of autoimmune diseases, such as rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, ulcerative colitis, moderate to severe chronic psoriasis and juvenile idiopathic arthritis.Mainly produced by activated macrophages (M1), Tumor necrosis factor (TNF, cachexin, cachectin, tumor necrosis factor alpha, TNFα) plays important roles in the regulation of immune cells. As a member of a group of cytokines that stimulate the acute phase reaction, TNF is involved in systemic inflammation and able to induce fever, apoptotic cell death, cachexia, inflammation, and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF expression could have serious impact in a variety of human diseases including Alzheimer's disease, cancer, major depression and inflammatory bowel disease.
IsotypeHuman IgG1 kappa
ImmunogenHuman TNF alpha
RecommendedIsotype Control(s)In Vivo Grade Recombinant Human IgG1 Kappa Isotype Control Antibody
Recommended Dilution Buffer1×PBS pH 7.0
ABclonal: - Human TNF alpha Monoclonal Antibody (YR0013)}

- Human TNF alpha Monoclonal Antibody (YR0013)

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human TNF-α (Research Grade Adalimumab Biosimilar) Monoclonal Antibody to TNF-α. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A donkey anti-human IgG HRP conjugate was used for detection.
ABclonal: - Human TNF alpha Monoclonal Antibody (YR0013)}

- Human TNF alpha Monoclonal Antibody (YR0013)

Determined through SPR assay, the Human Anti-Human TNF-α (Research Grade Adalimumab Biosimilar) Monoclonal Antibody is Capable of binding to Human TNF-α with an affinity constant of 0.1658 M.

* For research use only. Not for therapeutic or diagnostic purposes.