제품 > 항체 > 다중클론항체(pAb)

IFN gamma Rabbit pAb (A12450)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of Recombinant Human IFN-gamma Protein (RP01038), using IFN gamma Rabbit pAb (A12450) at 1:9000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 1ng/0.5ng/0.25ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - IFN gamma Rabbit pAb (A12450)

Immunohistochemistry analysis of paraffin-embedded Human lung using IFN gamma Rabbit pAb (A12450) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	IFN gamma Rabbit pAb
Catalog No.	A12450
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 24-161 of IFN gamma (NP_997468.1).
Sequence	VFGISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEE
Gene ID
Swiss Prot
Synonyms	IFG; IFI; IMD69; IFN gamma
Calculated MW	19kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Recombinant Human IFN-gamma Protein
Cellular location	Secreted.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus) IHC(Mus musculus, Homo sapiens, Mus musculus) ELISA(Mus musculus, Mus musculus ) IP(Homo sapiens, Mus musculus) IF(Mus musculus) IHC(Mus musculus) IF(Mus musculus)

Documents

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Research Area

IFN gamma Rabbit pAb images

Western blot - IFN gamma Rabbit pAb (A12450)

Immunohistochemistry - IFN gamma Rabbit pAb (A12450)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Cellular and molecular neurobiology

Application:

IF:

5.046

Species:

Mus musculus

PMID:

31140018

Title:

Neuroprotection of Resveratrol Against Focal Cerebral Ischemia/Reperfusion Injury in Mice Through a Mechanism Targeting Gut-Brain Axis.

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Neuroscience

Application:

IF:

3.59

Species:

Rattus norvegicus

PMID:

32081723

Title:

Sleep-restriction Inhibits Neurogenesis Through Decreasing the Infiltration of CD169+ Macrophages to Ischemic Brain After Stroke

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Life Sci

Application:

IF:

5.03

Species:

Mus musculus

PMID:

34384829

Title:

(R)-Ketamine ameliorates lethal inflammatory responses and multi-organ injury in mice induced by cecum ligation and puncture

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Mol Cancer

Application:

IF:

27.4

Species:

Mus musculus

PMID:

34906138

Title:

IRAK1-regulated IFN-γ signaling induces MDSC to facilitate immune evasion in FGFR1-driven hematological malignancies

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

J Neuroinflammation

Application:

IF:

8.322

Species:

Mus musculus

PMID:

34949194

Title:

RhANP attenuates endotoxin-derived cognitive dysfunction through subdiaphragmatic vagus nerve-mediated gut microbiota–brain axis

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Journal of medicinal chemistry

Application:

IHC

IF:

7.44

Species:

Mus musculus

PMID:

34110158

Title:

X-ray Structure-Guided Discovery of a Potent, Orally Bioavailable, Dual Human Indoleamine/Tryptophan 2,3-Dioxygenase (hIDO/hTDO) Inhibitor That Shows Activity in a Mouse Model of Parkinson's Disease

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

ACS applied materials & interfaces

Application:

IHC

IF:

10.38

Species:

Mus musculus

PMID:

35817627

Title:

Self-Assembly of a Multifunction DNA Tetrahedron for Effective Delivery of Aptamer PL1 and Pcsk9 siRNA Potentiate Immune Checkpoint Therapy for Colorectal Cancer - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF:

6.1

Species:

Mus musculus

PMID:

36563885

Title:

Exploring the potential molecular mechanism of trastuzumab-induced cardiotoxicity based on RNA sequencing and bioinformatics analysis

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Psychopharmacology

Application:

ELISA

IF:

4.41

Species:

Mus musculus

PMID:

34313805

Title:

(R)-Ketamine attenuates LPS-induced endotoxin-derived delirium through inhibition of neuroinflammation

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Biomedicines

Application:

IF:

4.75

Species:

Homo sapiens

PMID:

36979934

Title:

Aberrant Activation of the STING-TBK1 Pathway in γδ T Cells Regulates Immune Responses in Oral Lichen Planus - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Bioactive materials

Application:

ELISA

IF:

16.87

Species:

Mus musculus

PMID:

37408796

Title:

An ischemic area-targeting, peroxynitrite-responsive, biomimetic carbon monoxide nanogenerator for preventing myocardial ischemia-reperfusion injury - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Cancer cell international

Application:

IHC

IF:

6.42

Species:

Mus musculus

PMID:

37779195

Title:

HK3 stimulates immune cell infiltration to promote glioma deterioration - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Nutrients

Application:

IHC

IF:

6.7

Species:

Mus musculus

PMID:

37960350

Title:

1,25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Mechanisms of ageing and development

Application:

IF:

5.49

Species:

Mus musculus

PMID:

38007051

Title:

Protective Effects of Apigenin on the Brain Transcriptome with Aging

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Cancer immunology, immunotherapy : CII

Application:

WB, IP

IF:

6.63

Species:

Homo sapiens, Mus musculus

PMID:

36688994

Title:

Radiotherapy enhances CXCR3 (high) CD8 (+) T cells activation through inducing IFN-mediated ICAM-1 expression in lung cancer cells

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Pharmaceutics

Application:

IHC, IF

IF:

6.52

Species:

Mus musculus

PMID:

36145710

Title:

Biodegradation of HA and -TCP Ceramics Regulated by T-Cells - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IHC

IF:

6.2

Species:

Homo sapiens, Mus musculus

PMID:

36430250

Title:

Fascaplysin Induces Apoptosis and Ferroptosis, and Enhances Anti-PD-1 Immunotherapy in Non-Small Cell Lung Cancer (NSCLC) by Promoting PD-L1 Expression - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Acta pharmacologica Sinica

Application:

WB, IHC, IF

IF:

7.16

Species:

Mus musculus

PMID:

37749237

Title:

Rosmarinic acid in combination with ginsenoside Rg1 suppresses colon cancer metastasis via co-inhition of COX-2 and PD1/PD-L1 signaling axis

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.3

Species:

Mus musculus

PMID:

38715061

Title:

Neurons upregulate PD-L1 via IFN/STAT1/IRF1 to alleviate damage by CD8+ T cells in cerebral malaria - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

International journal of pharmaceutics

Application:

IHC

IF:

5.8

Species:

Mus musculus

PMID:

38565406

Title:

A novel FK506-loading mesoporous silica nanoparticle homing to lymph nodes for transplant rejection treatment

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

International immunopharmacology

Application:

IHC

IF:

5.71

Species:

Homo sapiens,Mus musculus

PMID:

36461605

Title:

New Lactobacillus plantarum membrane proteins (LpMPs) towards oral anti-inflammatory agents against dextran sulfate sodium-induced colitis

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Virchows Archiv : an international journal of pathology

Application:

IHC

IF:

3.5

Species:

Homo sapiens

PMID:

38748263

Title:

Correlation of High-Grade Osteosarcoma Response to Chemotherapy with Enhanced Tissue Immunological Response: Analysis of CD95R, IFN-, Catalase, Hsp70, and VEGF - PMC

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

International immunopharmacology

Application:

IHC

IF:

5.6

Species:

Mus musculus

PMID:

38909500

Title:

APLNR inhibited nasopharyngeal carcinoma growth and immune escape by downregulating PD-L1

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Bioactive materials

Application:

IF:

18.9

Species:

Rattus norvegicus

PMID:

39022185

Title:

A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder

References for IFN gamma Rabbit pAb (A12450)

Product:

IFN gamma Rabbit pAb

Journal:

Human vaccines & immunotherapeutics

Application:

IF:

4.8

Species:

Mus musculus

PMID:

39327639