제품 > 항체 > 다중클론항체(pAb)

IL10 Rabbit pAb (A2171)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using IL10 Rabbit pAb (A2171) at 1:800 dilution. Raw264. 7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - IL10 Rabbit pAb (A2171)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using IL10 Rabbit pAb (A2171) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Review

Overview

Product name	IL10 Rabbit pAb
Catalog No.	A2171
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 19-178 of human IL10 (NP_000563.1).
Sequence	SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN
Gene ID
Swiss Prot
Synonyms	CSIF; TGIF; GVHDS; IL-10; IL10A; IL10
Calculated MW	21kDa
Observed MW	18kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:100 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	RAW264.7 treated by LPS and Brefeldin A
Cellular location	Secreted.
Customer validation	(Mouse embryonal fibroblast cell) WB(Rattus norvegicus, Cynoglossus semilaevis, Mus musculus, Homo sapiens, Mus musculus, Canis lupus familiaris, Capra hircus) IF(Homo sapiens, Mus musculus) IHC(Rattus norvegicus) IHC(Homo sapiens) FC(Canis lupus familiaris) IF(Capra hircus) PCR(Rattus norvegicus) WB(Rattus norvegicus, Mus musculus) IHC(Mus musculus) Other(Mus musculus)

Documents

Certificate of Compliance

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Research Area

IL10 Rabbit pAb images

Western blot - IL10 Rabbit pAb (A2171)

Immunohistochemistry - IL10 Rabbit pAb (A2171)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2171

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Reviews List

Product Protocols

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

5.923

Species:

Rattus norvegicus

PMID:

30678337

Title:

Ferulic Acid Improves Depressive-Like Behavior in Prenatally-Stressed Offspring Rats via Anti-Inflammatory Activity and HPA Axis.

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Veterinary microbiology

Application:

IF:

3.293

Species:

Cynoglossus semilaevis

PMID:

30827402

Title:

In vivo cellular and molecular study on duck spleen infected by duck Tembusu virus.

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.932

Species:

Mus musculus

PMID:

30952101

Title:

The role of netrin-1 in the mouse cornea during Aspergillus fumigatus infection.

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Cellular and molecular neurobiology

Application:

IF:

5.046

Species:

Mus musculus

PMID:

31140018

Title:

Neuroprotection of Resveratrol Against Focal Cerebral Ischemia/Reperfusion Injury in Mice Through a Mechanism Targeting Gut-Brain Axis.

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

4.379

Species:

Mus musculus

PMID:

32034203

Title:

TNF-α and INF-γ primed canine stem cell-derived extracellular vesicles alleviate experimental murine colitis

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Molecular Therapy-Nucleic Acids

Application:

IF:

8.886

Species:

Homo sapiens

PMID:

32059338

Title:

Identification of the Regulatory Role of lncRNA SNHG16 in Myasthenia Gravis by Constructing a Competing Endogenous RNA Network.

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Brain Res Bull

Application:

IF:

4.077

Species:

Mus musculus

PMID:

32987101

Title:

Treadmill exercise mitigates neuroinflammation and increases BDNF via activation of SIRT1 signaling in a mouse model of T2DM

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Reprod Biol Endocrinol

Application:

IF:

5.211

Species:

Mus musculus

PMID:

33472656

Title:

Leptin and inflammatory factors play a synergistic role in the regulation of reproduction in male mice through hypothalamic kisspeptin-mediated energy balance

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

J Mol Cell Cardiol

Application:

IF:

Species:

Homo sapiens

PMID:

32445843

Title:

miR-374b-5p is increased in deep vein thrombosis and negatively targets IL-10

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Int J Endocrinol

Application:

IHC

IF:

3.25

Species:

Rattus norvegicus

PMID:

34422046

Title:

Effects of Selenium Supplement on B Lymphocyte Activity in Experimental Autoimmune Thyroiditis Rats

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Life Sci

Application:

IF:

5.03

Species:

Mus musculus

PMID:

34384829

Title:

(R)-Ketamine ameliorates lethal inflammatory responses and multi-organ injury in mice induced by cecum ligation and puncture

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

J Ethnopharmacol

Application:

IF:

4.36

Species:

Homo sapiens

PMID:

33716082

Title:

Taraxacum mongolicum extract inhibited malignant phenotype of triple-negative breast cancer cells in tumor-associated macrophages microenvironment through suppressing IL-10 / STAT3 / PD-L1 signaling pathways

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Physiol Rep

Application:

IF:

Species:

Mus musculus

PMID:

34435466

Title:

Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Shock

Application:

IF:

3.454

Species:

Rattus norvegicus

PMID:

33927140

Title:

Vagus Nerve Stimulation Protects Enterocyte Glycocalyx After Hemorrhagic Shock Via the Cholinergic Anti-Inflammatory Pathway

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

IF:

2.65

Species:

Rattus norvegicus

PMID:

36212957

Title:

The Mitigatory Effect of Shen-Qi Compound on the Diabetic Thoracic Aortic Complications through Inhibiting the Inflammatory Microenvironment by miR-223-3p/RBP-J/IRF8 Axis - PMC

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Advanced healthcare materials

Application:

IHC

IF:

11.09

Species:

Rattus norvegicus

PMID:

34779582

Title:

A Hierarchical‐Structured Mineralized Nanofiber Scaffold with Osteoimmunomodulatory and Osteoinductive Functions for Enhanced Alveolar Bone Regeneration

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Cancer gene therapy

Application:

IF:

5.85

Species:

Homo sapiens

PMID:

36434176

Title:

PDPN contributes to constructing immunosuppressive microenvironment in IDH wildtype glioma

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Immunological investigations

Application:

IF:

3.04

Species:

Mus musculus

PMID:

36731128

Title:

All-Trans Retinoic Acid Promotes M2 Macrophage Polarization in Vitro by Activating the p38MAPK/STAT6 Signaling Pathway

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Cytokine

Application:

IF:

3.92

Species:

Rattus norvegicus

PMID:

36716676

Title:

Majoon chobchini reinstates PDL-1 expression and blocks dendritic cell-T helper 17 pathogenic axis in rheumatoid arthritis animal model

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Gut microbes

Application:

WB, IHC

IF:

9.434

Species:

Homo sapiens

PMID:

34606408

Title:

Two ST11 Klebsiella pneumoniae strains exacerbate colorectal tumorigenesis in a colitis-associated mouse model

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Cell Proliferation

Application:

IF:

8.755

Species:

Homo sapiens, Mus musculus

PMID:

31418947

Title:

Inhibition of CREB-mediated ZO-1 and Activation of NF-B-induced IL-6 by Colonic Epithelial MCT4 Destroys Intestinal Barrier Function.

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

PLoS One

Application:

WB, FC

IF:

3.752

Species:

Canis lupus familiaris

PMID:

34807933

Title:

Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

PLoS pathogens

Application:

WB, IF

IF:

7.46

Species:

Capra hircus

PMID:

36084159

Title:

Extracellular vesicles derived from PPRV-infected cells enhance signaling lymphocyte activation molecular (SLAM) receptor expression and facilitate virus infection - PMC

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Canadian journal of gastroenterology & hepatology

Application:

IF:

2.6

Species:

Rattus norvegicus

PMID:

38187112

Title:

Ghrelin/GHSR Axis Induced M2 Macrophage and Alleviated Intestinal Barrier Dysfunction in a Sepsis Rat Model by Inactivating E2F1/NF-B Signaling - PMC

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Brain research bulletin

Application:

IF:

3.71

Species:

Mus musculus

PMID:

38145759

Title:

Betaine eliminates CFA-induced depressive-like behaviour in mice may be through inhibition of microglia and astrocyte activation and polarization

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Journal of molecular neuroscience

Application:

IF:

2.866

Species:

Mus musculus

PMID:

30484061

Title:

Non-invasive Vagus Nerve Stimulation Protects Against Cerebral Ischemia/Reperfusion Injury and Promotes Microglial M2 Polarization Via Interleukin-17A Inhibition.

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Molecular neurobiology

Application:

PCR, WB

IF:

5.1

Species:

Rattus norvegicus

PMID:

38954253

Title:

Huanglian Wendan Decoction Improves Insomnia in Rats by Regulating BDNF/TrkB Signaling Pathway Through Gut Microbiota-Mediated SCFAs and Affecting

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Rattus norvegicus

PMID:

39144974

Title:

Peimine promotes microglial polarization to the M2 phenotype to attenuate drug-resistant epilepsy through suppressing the TLR4/NF-B/HIF-1 signaling pathway in a rat model and in BV-2 microglia - PMC

References for IL10 Rabbit pAb (A2171)

Product:

IL10 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Rattus norvegicus

PMID:

39295999

Title: