제품 > 항체 > 다중클론항체(pAb)

IL18 Rabbit pAb (A16737)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using IL18 Rabbit pAb (A16737) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - IL18 Rabbit pAb (A16737)

Immunofluorescence analysis of C6 cells using IL18 Rabbit pAb (A16737) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using IL18 Rabbit pAb (A16737) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using IL18 Rabbit pAb (A16737) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	IL18 Rabbit pAb
Catalog No.	A16737
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 31-130 of human IL18 (NP_001553.1).
Sequence	ENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKD
Gene ID
Swiss Prot
Synonyms	IGIF; IL-18; IL-1g; IL1F4; IL18
Calculated MW	22kDa
Observed MW	22kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	DU145, HeLa, Raji(Negative control)
Cellular location	Secreted.
Customer validation	WB(Rattus norvegicus, Mus musculus, Homo sapiens, Danio rerio, Sus scrofa, Other) ELISA(Homo sapiens) IHC(Mus musculus) Other(Mus musculus)

Documents

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Research Area

IL18 Rabbit pAb images

Western blot - IL18 Rabbit pAb (A16737)

Immunofluorescence - IL18 Rabbit pAb (A16737)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Pharmacological research

Application:

IF:

9.1

Species:

Rattus norvegicus

PMID:

31276771

Title:

Pharmacological inhibition of the NLRP3 inﬂammasome as a potential target for cancer-induced bone pain.

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Toxicology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

32014472

Title:

NLRP3 inflammasome inhibition attenuates subacute neurotoxicity induced by acrylamide in vitro and in vivo

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Atherosclerosis

Application:

IF:

4.9

Species:

Mus musculus

PMID:

33321327

Title:

Targeting HDAC6 attenuates nicotine-induced macrophage pyroptosis via NF-κB/NLRP3 pathway

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

J Inorg Biochem

Application:

IF:

3.8

Species:

Mus musculus

PMID:

33607539

Title:

Puerarin induces Nrf2 as a cytoprotective mechanism to prevent cadmium-induced autophagy inhibition and NLRP3 inflammasome activation in AML12 hepatic cells

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Molecules

Application:

IF:

4.6

Species:

Mus musculus

PMID:

32646056

Title:

Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Immunobiology

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

34469785

Title:

Long non-coding RNA RP11-490M8.1 inhibits lipopolysaccharide-induced pyroptosis of human umbilical vein endothelial cells via the TLR4/NF-κB pathway

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Biochem Cell Biol

Application:

IF:

2.4

Species:

Mus musculus

PMID:

33939927

Title:

CLEC5A knockdown protects against the cardiac dysfunction after Myocardial infarction by suppressing macrophage polarization, NLRP3 inflammasome activation and pyroptosis

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Toxicol Lett

Application:

IF:

2.9

Species:

Danio rerio

PMID:

34718095

Title:

Aurantio-obtusin induces hepatotoxicity through activation of NLRP3 inflammasome signaling

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Neuroscience

Application:

IF:

2.9

Species:

Rattus norvegicus

PMID:

36067951

Title:

Inhibition of miR-423-5p exerts neuroprotective effects in an experimental rat model of cerebral ischemia/reperfusion injury

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Food & function

Application:

IF:

5.1

Species:

Mus musculus

PMID:

35260874

Title:

Lycopene Ameliorates Atrazine-Induced Pyroptosis in Spleen via Suppressing the Ox-mtDNA/Nlrp3 Inflammasome Pathway

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Journal of food and drug analysis

Application:

IF:

2.6

Species:

Homo sapiens

PMID:

37335159

Title:

The inhibitory effect of quercetin-3-glucuronide on pulmonary injury in vitro and in vivo - PMC

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Molecular medicine reports

Application:

IF:

3.4

Species:

Mus musculus

PMID:

37711056

Title:

Dapagliflozin alleviates renal podocyte pyroptosis via regulation of the HO-1/NLRP3 axis - PMC

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Infection and drug resistance

Application:

IF:

2.9

Species:

Sus scrofa

PMID:

37404255

Title:

18-Glycyrrhetinic Acid Alleviates P. multocida-Induced Vascular Endothelial Inflammation by PARP1-Mediated NF-B and HMGB1 Signalling Suppression in PIEC Cells - PMC

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF:

5.3

Species:

Rattus norvegicus

PMID:

37604293

Title:

Nicotinic acid attenuates experimental non-alcoholic steatohepatitis by inhibiting the NLRP3 inflammasome/pyroptosis pathway

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Experimental and therapeutic medicine

Application:

IF:

2.4

Species:

Mus musculus

PMID:

37602308

Title:

MCC950 improves lipopolysaccharide-induced systemic inflammation in mice by relieving pyroptosis in blood neutrophils - PMC

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Molecular neurobiology

Application:

ELISA

IF:

4.6

Species:

Homo sapiens

PMID:

36324051

Title:

Acrylamide Induces Neurotoxicity in SH-SY5Y Cells via NLRP3-mediated Pyroptosis

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.7

Species:

Rattus norvegicus

PMID:

38368794

Title:

Shen-Shuai-II-Recipe inhibits tubular inflammation by PPAR-mediated fatty acid oxidation to attenuate fibroblast activation in fibrotic kidneys

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Toxicological sciences

Application:

WB, IHC

IF:

3.4

Species:

Mus musculus

PMID:

31070765

Title:

Blockade of the NLRP3/Caspase-1 Axis Ameliorates Airway Neutrophilic Inflammation in a Toluene Diisocyanate-Induced Murine Asthma Model.

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

J Neuroinflammation

Application:

IF:

9.6

Species:

Rattus norvegicus

PMID:

34362408

Title:

Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia-reperfusion injury via astrocyte NF-B/NLRP3 inflammasome pathway : HSPA8 inhibition protects spinal ischemia-reperfusion injury

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Biomolecules

Application:

IF:

5.5

Species:

Mus musculus

PMID:

35883482

Title:

Cold Stress Induced Liver Injury of Mice through Activated NLRP3/Caspase-1/GSDMD Pyroptosis Signaling Pathway - PMC

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Biomed Pharmacother

Application:

IF:

6.9

Species:

Mus musculus

PMID:

34474337

Title:

MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Acta tropica

Application:

IF:

2.1

Species:

Mus musculus

PMID:

35963313

Title:

Toxoplasma gondii rhoptry protein (TgROP18) enhances the expression of pro-inflammatory factor in LPS/IFN--induced murine BV2 microglia cells via NF-B signal

References for IL18 Rabbit pAb (A16737)

Product:

IL18 Rabbit pAb

Journal:

Brain injury

Application:

IF:

1.5

Species:

Other

PMID:

38711413