제품 > 항체 > 다중클론항체(pAb)

IL6 Rabbit pAb (A0286)

Reviews (4)Publications (154) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:IL6 Rabbit pAb
ABclonal:Western blot - IL6 Rabbit pAb (A0286)

Western blot analysis of lysates from Recombinant Human IL6 Protein (RP02236) using IL6 Rabbit pAb (A0286) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng, 5ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - IL6 Rabbit pAb (A0286)

Immunofluorescence analysis of NIH/3T3 cells using IL6 Rabbit pAb (A0286) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)

Overview

Product nameIL6 Rabbit pAb
Catalog No.A0286
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 30-212 of human IL6 (NP_000591.1).
SequenceVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM
Gene ID
Swiss Prot
SynonymsCDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2; IL6
Calculated MW24kDa
Observed MW23-30kDa/19, 22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRecombinant Human IL6 Protein, THP-1, HL-60, U-937, Jurkat(阴性), mouse thymus, mouse spleen, rat thymus
Cellular locationSecreted.
Customer validation

(Mouse embryonal fibroblast cell, Human colon cancer cell, Mouse skeletal muscle)

WB(Mus musculus, Rattus norvegicus, Cynoglossus semilaevis, Homo sapiens, Oryctolagus cuniculus, Bos taurus, Mus musculus, Rattus norvegicus, Gallus gallus, Other)

IHC(Homo sapiens, Mus musculus, Rattus norvegicus, Mus musculus)

IHC(Rattus norvegicus, Mus musculus, Mus musculus )

IF(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus)

IF(Mus musculus, Rattus norvegicus)

IHC(Mus musculus, Homo sapiens)

WB(Mus musculus, Rattus norvegicus)

ELISA(Mus musculus, Ovis aries)

WB(Mus musculus)

Other(Rattus norvegicus, Mus musculus)

WB (Rattus norvegicus)

PCR(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - IL6 Rabbit pAb (A0286)}

Western blot - IL6 Rabbit pAb (A0286)

Western blot analysis of lysates from Recombinant Human IL6 Protein (RP02236) using IL6 Rabbit pAb (A0286) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng, 5ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - IL6 Rabbit pAb (A0286)}

Immunofluorescence - IL6 Rabbit pAb (A0286)

Immunofluorescence analysis of NIH/3T3 cells using IL6 Rabbit pAb (A0286) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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ELISA 키트 (3)

Human IL-6 ELISA Kit
RK00004

Human IL-6 ELISA Kit

Human IL-6 FAST ELISA Kit
RK04769

Human IL-6 FAST ELISA Kit

Human IL-6 HP ELISA Kit
RK04786HP

Human IL-6 HP ELISA Kit

항체 (4)

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WBELISA

Reactivity:Human, Mouse, Rat

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WBELISA

Reactivity:Human

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A14687

WBELISA

Reactivity:Human, Mouse, Rat

IL6 Rabbit pAb

IL6 Rabbit mAb
A24913

WBIF/ICCELISA

Reactivity:Human

IL6 Rabbit mAb

재조합 단백질 (1)

Recombinant Human IL-6 Protein
RP00201

Tags:C-His

Expressed Host:HEK293 cells

Recombinant Human IL-6 Protein

제품 (8)

Rabbit anti-Human IL-6 mAb (CAP)
RM17601

Rabbit anti-Human IL-6 mAb (CAP)

Rabbit anti-Human IL-6 mAb (CAP)
RM17629

Rabbit anti-Human IL-6 mAb (CAP)

Rabbit anti-Human IL-6 mAb (DET)
RM17652

Rabbit anti-Human IL-6 mAb (DET)

Rabbit anti-Human IL-6 mAb (CAP)
RM17650

Rabbit anti-Human IL-6 mAb (CAP)

Rabbit anti-Human IL-6 mAb (DET)
RM17630

Rabbit anti-Human IL-6 mAb (DET)

Rabbit anti-Human IL-6 mAb (DET)
RM17631

Rabbit anti-Human IL-6 mAb (DET)

Rabbit anti-Human IL-6 mAb (DET)
RM17651

Rabbit anti-Human IL-6 mAb (DET)

Rabbit anti-Human IL-6 mAb (DET)
RM17602

Rabbit anti-Human IL-6 mAb (DET)

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)