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IP3R Rabbit mAb (A4436)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - IP3R Rabbit mAb (A4436)

Western blot analysis of lysates from Mouse brain, using IP3R Rabbit mAb (A4436) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A4436)

Immunohistochemistry analysis of paraffin-embedded Rat brain using IP3R Rabbit mAb (A4436) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A4436)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using IP3R Rabbit mAb (A4436) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - IP3R Rabbit mAb (A4436)

Immunofluorescence analysis of paraffin-embedded rat brain using IP3R Rabbit mAb (A4436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IP3R Rabbit mAb (A4436)

Immunofluorescence analysis of paraffin-embedded mouse brain using IP3R Rabbit mAb (A4436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIP3R Rabbit mAb
Catalog No.A4436
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1007
This gene encodes an intracellular receptor for inositol 1, 4, 5-trisphosphate. Upon stimulation by inositol 1, 4, 5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 2200-2300 of human IP3R (Q14643).
SequenceHTAQIEIVRLDRTMEQIVFPVPSICEFLTKESKLRIYYTTERDEQGSKINDFFLRSEDLFNEMNWQKKLRAQPVLYWCARNMSFWSSISFNLAVLMNLLVA
Gene ID
Swiss Prot
SynonymsACV; CLA4; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1; PPP1R94
Calculated MW314kDa
Observed MW320kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:100 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain
Cellular locationEndoplasmic reticulum membrane, Cytoplasm, perinuclear region, Cytoplasm, perinuclear region.
Customer validation

WB(Sus scrofa, Gallus gallus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - IP3R Rabbit mAb (A4436)}

Western blot - IP3R Rabbit mAb (A4436)

Western blot analysis of lysates from Mouse brain, using IP3R Rabbit mAb (A4436) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A4436)}

Immunohistochemistry - IP3R Rabbit mAb (A4436)

Immunohistochemistry analysis of paraffin-embedded Rat brain using IP3R Rabbit mAb (A4436) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A4436)}

Immunohistochemistry - IP3R Rabbit mAb (A4436)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using IP3R Rabbit mAb (A4436) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - IP3R Rabbit mAb (A4436)}

Immunofluorescence - IP3R Rabbit mAb (A4436)

Immunofluorescence analysis of paraffin-embedded rat brain using IP3R Rabbit mAb (A4436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IP3R Rabbit mAb (A4436)}

Immunofluorescence - IP3R Rabbit mAb (A4436)

Immunofluorescence analysis of paraffin-embedded mouse brain using IP3R Rabbit mAb (A4436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)