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ITCH Rabbit pAb (A17389)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ITCH Rabbit pAb (A17389)

Western blot analysis of lysates from 293T cells, using ITCH Rabbit pAb (A17389) at dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - ITCH Rabbit pAb (A17389)

Western blot analysis of various lysates using ITCH Rabbit pAb (A17389) at dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - ITCH Rabbit pAb (A17389)

Immunohistochemistry analysis of paraffin-embedded rat brain using ITCH Rabbit pAb (A17389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ITCH Rabbit pAb (A17389)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using ITCH Rabbit pAb (A17389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ITCH Rabbit pAb (A17389)

Immunohistochemistry analysis of paraffin-embedded human placenta using ITCH Rabbit pAb (A17389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ITCH Rabbit pAb (A17389)

Immunohistochemistry analysis of paraffin-embedded mouse brain using ITCH Rabbit pAb (A17389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameITCH Rabbit pAb
Catalog No.A17389
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-155 of ITCH (NP_001244066.1).
SequenceMSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETT
Gene ID
Swiss Prot
SynonymsAIF4; AIP4; ADMFD; NAPP1; ITCH
Calculated MW103kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, NIH/3T3, C6, Mouse lung
Cellular locationcell cortex, cytoplasm, cytoplasmic vesicle, cytosol, extracellular exosome, nucleoplasm, plasma membrane

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - ITCH Rabbit pAb (A17389)}

Western blot - ITCH Rabbit pAb (A17389)

Western blot analysis of lysates from 293T cells, using ITCH Rabbit pAb (A17389) at dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - ITCH Rabbit pAb (A17389)}

Western blot - ITCH Rabbit pAb (A17389)

Western blot analysis of various lysates using ITCH Rabbit pAb (A17389) at dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - ITCH Rabbit pAb (A17389)}

Immunohistochemistry - ITCH Rabbit pAb (A17389)

Immunohistochemistry analysis of paraffin-embedded rat brain using ITCH Rabbit pAb (A17389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ITCH Rabbit pAb (A17389)}

Immunohistochemistry - ITCH Rabbit pAb (A17389)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using ITCH Rabbit pAb (A17389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ITCH Rabbit pAb (A17389)}

Immunohistochemistry - ITCH Rabbit pAb (A17389)

Immunohistochemistry analysis of paraffin-embedded human placenta using ITCH Rabbit pAb (A17389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ITCH Rabbit pAb (A17389)}

Immunohistochemistry - ITCH Rabbit pAb (A17389)

Immunohistochemistry analysis of paraffin-embedded mouse brain using ITCH Rabbit pAb (A17389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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