Basic Information
| Clone | 4F17m |
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| Molecular Weight | 150 kDa |
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| Endotoxin | <1EU/mg (<0.001EU/μg)Determined by LAL gel clotting assay |
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| Sterility | 0.2 μm filtration |
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| Aggregation | <5% Determined by SECP |
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| Purity | >95% Determined by SDS-PAGE |
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Product Information
| Production | Purified from cell culture supernatant in an animal-free facility |
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| Purification | Protein A or G purification |
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| Storage | 2 - 8°C for up to 4 weeks and -80°C for long term storage (Avoid repeated freezing and thawing) |
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Target Background
Isotype control antibodies are used as negative controls of primary antibodies to check presence of non-specific binding to FC receptors on the cell surface, other cellular proteins, carbohydrates, and lipids. Thus, the isotype control antibody normally matches the host species and isotype, including light chains (such as human IgG4 S228P kappa antibody), concentration, and conjugation format of the experimental primary antibody. The ideal human isotype control antibody has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG constant regions.
Immunogen Information
| Isotype | Human IgG4 kappa |
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| Recommended Dilution Buffer | 1×PBS pH 7.0 |
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* For research use only. Not for therapeutic or diagnostic purposes.
