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[KD Validated] ATG7 Rabbit mAb (A19604)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KD Validated] ATG7 Rabbit mAb (A19604) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from wild type(WT) and ATG7 knockdown (KD) HeLa cells using [KD Validated] ATG7 Rabbit mAb (A19604) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KD Validated] ATG7 Rabbit mAb (A19604)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] ATG7 Rabbit mAb (A19604) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KD Validated] ATG7 Rabbit mAb (A19604) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] ATG7 Rabbit mAb (A19604) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] ATG7 Rabbit mAb (A19604) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] ATG7 Rabbit mAb (A19604) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name	[KD Validated] ATG7 Rabbit mAb
Catalog No.	A19604
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0083

Background

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Atg7(Apg7) (O95352).
Sequence	MAAATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTAD
Gene ID
Swiss Prot
Synonyms	GSA7; [KD Validated] ATG7
Calculated MW	78kDa
Observed MW	77kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IHC-P 1:200 - 1:800 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HeLa, THP-1, 293T, Mouse brain, Mouse lung, Mouse spleen, Rat testis
Cellular location	Cytoplasm, Preautophagosomal structure.
Customer validation	IP(Homo sapiens) WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Other) WB(Other)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] ATG7 Rabbit mAb images

Western blot - [KD Validated] ATG7 Rabbit mAb (A19604)

Immunohistochemistry - [KD Validated] ATG7 Rabbit mAb (A19604)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KD Validated] ATG7 Rabbit mAb (A19604)

Product:

[KD Validated] ATG7 Rabbit mAb

Journal:

Cell Death Dis

Application:

IF:

8.469

Species:

Homo sapiens

PMID:

33637691

Title:

SIRT1 attenuates sepsis-induced acute kidney injury via Beclin1 deacetylation-mediated autophagy activation.

References for [KD Validated] ATG7 Rabbit mAb (A19604)

Product:

[KD Validated] ATG7 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.67

Species:

Mus musculus

PMID:

35739966

Title:

Alanyl-Glutamine Protects against Lipopolysaccharide-Induced Liver Injury in Mice via Alleviating Oxidative Stress, Inhibiting Inflammation, and Regulating Autophagy

References for [KD Validated] ATG7 Rabbit mAb (A19604)

Product:

[KD Validated] ATG7 Rabbit mAb

Journal:

The international journal of biochemistry & cell biology

Application:

IF:

5.65

Species:

Homo sapiens

PMID:

37884171

Title:

The bcl6 corepressor mutation regulates the progression and transformation of myelodysplastic syndromes by repressing the autophagy flux

References for [KD Validated] ATG7 Rabbit mAb (A19604)

Product:

[KD Validated] ATG7 Rabbit mAb

Journal:

IUBMB life

Application:

IF:

4.7

Species:

Homo sapiens

PMID:

37818680

Title:

Nacetylneuraminic acid modulates SQSTM1/p62 sialyationmediated ubiquitination degradation contributing to vascular endothelium dysfunction in experimental

References for [KD Validated] ATG7 Rabbit mAb (A19604)

Product:

[KD Validated] ATG7 Rabbit mAb

Journal:

Autophagy

Application:

IF:

13.39

Species:

Homo sapiens

PMID:

37471054

Title:

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

References for [KD Validated] ATG7 Rabbit mAb (A19604)

Product:

[KD Validated] ATG7 Rabbit mAb

Journal:

EMBO reports

Application:

IF:

9.07

Species:

Homo sapiens

PMID:

37846507

Title:

An ULK1/2PXN mechanotransduction pathway suppresses breast cancer cell migration

References for [KD Validated] ATG7 Rabbit mAb (A19604)

Product:

[KD Validated] ATG7 Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

17.52

Species:

Rattus norvegicus

PMID:

36808838

Title:

Suppressing Mesenchymal Stromal Cell Ferroptosis Via Targeting a MetabolismEpigenetics Axis Corrects their Poor Retention and Insufficient Healing Benefits in

References for [KD Validated] ATG7 Rabbit mAb (A19604)

Product:

[KD Validated] ATG7 Rabbit mAb

Journal:

Nature communications

Application:

IF, WB

IF:

16.6

Species:

Other

PMID: