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[KD Validated] NQO1 Rabbit mAb (A19586)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using [KD Validated] NQO1 Rabbit mAb (A19586) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from wild type (WT) and NQO1 knockdown (KD) HeLa cells, using [KD Validated] NQO1 Rabbit mAb (A19586) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KD Validated] NQO1 Rabbit mAb (A19586)

Confocal imaging of paraffin-embedded Human stomach tissue using [KD Validated] NQO1 Rabbit mAb (A19586, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Mouse stomach tissue using [KD Validated] NQO1 Rabbit mAb (A19586, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Rat stomach tissue using [KD Validated] NQO1 Rabbit mAb (A19586, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product name	[KD Validated] NQO1 Rabbit mAb
Catalog No.	A19586
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC56753

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NQO1 (P15559).
Sequence	MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIF
Gene ID
Swiss Prot
Synonyms	DTD; QR1; DHQU; DIA4; NMOR1; NMORI; [KD Validated] NQO1
Calculated MW	31kDa
Observed MW	31kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:20000 - 1:120000 IF/ICC 1:300 - 1:1200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, Mouse Stomach, Rat Lung, Rat Stomach
Cellular location	Cytoplasm.
Customer validation	WB(Mus musculus, Homo sapiens, Vaccinium dunalianum, Rattus norvegicus, Sus scrofa, Bos taurus) IHC(Mus musculus) RT-qPCR(Homo sapiens) WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] NQO1 Rabbit mAb images

Western blot - [KD Validated] NQO1 Rabbit mAb (A19586)

Immunofluorescence - [KD Validated] NQO1 Rabbit mAb (A19586)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Frontiers in Immunology

Application:

IF:

7.561

Species:

Mus musculus

PMID:

32174909

Title:

PL201, a Reported Rhamnoside Against Alzheimer's Disease Pathology, Alleviates Neuroinflammation and Stimulates Nrf2 Signaling

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Free Radic Biol Med

Application:

IF:

7.376

Species:

Homo sapiens

PMID:

33450381

Title:

Downregulation of CD151 induces oxidative stress and apoptosis in trophoblast cells via inhibiting ERK/Nrf2 signaling pathway in preeclampsia

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Int J Nanomedicine

Application:

IF:

6.4

Species:

Homo sapiens

PMID:

33664571

Title:

Copper Nanoparticles Induce Oxidative Stress via the Heme Oxygenase 1 Signaling Pathway in vitro Studies

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Food Sci Biotechnol

Application:

IF:

2.39

Species:

Mus musculus

PMID:

34471563

Title:

Anwulignan alleviates d-galactose induced renal damage by regulating Nrf2/ARE signaling pathway in mice

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Ecotoxicol Environ Saf

Application:

IF:

6.291

Species:

Homo sapiens

PMID:

34619472

Title:

In vitro evaluation of nanoplastics using human lung epithelial cells, microarray analysis and co-culture model

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

J Ethnopharmacol

Application:

IF:

4.36

Species:

Mus musculus

PMID:

34662665

Title:

Chinese sumac (Rhus chinensis Mill.) fruits alleviate indomethacin-induced gastric ulcer in mice by improving oxidative stress, inflammation and apoptosis

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Food Chem

Application:

IF:

7.514

Species:

Vaccinium dunalianum

PMID:

34896954

Title:

Phenolic constituents, antioxidant activity and neuroprotective effects of ethanol extracts of fruits, leaves and flower buds from Vaccinium dunalianum Wight

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

34813638

Title:

Gastroprotective effect and mechanisms of Chinese sumac fruits (Rhus chinensis Mill.) on ethanol-induced gastric ulcers in mice

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

European journal of medicinal chemistry

Application:

IF:

7.08

Species:

Mus musculus

PMID:

35987018

Title:

A multi-target directed ligands strategy for the treatment of Alzheimer's disease: Dimethyl fumarate plus Tranilast modified Dithiocarbate as AChE inhibitor and Nrf2 …

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

PloS one

Application:

IHC

IF:

3.75

Species:

Mus musculus

PMID:

35696363

Title:

Nrf2 deficiency causes hepatocyte dedifferentiation and reduced albumin production in an experimental extrahepatic cholestasis model - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IF:

5.83

Species:

Mus musculus

PMID:

36094000

Title:

Intranasal administration of β‐1, 3‐galactosyltransferase 2 confers neuroprotection against ischemic stroke by likely inhibiting oxidative stress and NLRP3 …

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Acta pharmaceutica Sinica. B

Application:

IF:

14.9

Species:

Mus musculus

PMID:

35127378

Title:

Astaxanthine attenuates cisplatin ototoxicity in vitro and protects against cisplatin-induced hearing loss in vivo

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Toxins

Application:

IF:

5.07

Species:

Rattus norvegicus

PMID:

35737028

Title:

Role of Nrf2 Nucleus Translocation in Beauvericin-Induced Cell Damage in Rat Hepatocytes

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Bioorganic & medicinal chemistry

Application:

IF:

3.46

Species:

Rattus norvegicus

PMID:

36580744

Title:

4Dual functional antioxidant and butyrylcholinesterase inhibitors for the treatment of Alzheimer's disease: Design, synthesis and evaluation of novel melatonin …

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Nutrients

Application:

IF:

6.7

Species:

Mus musculus

PMID:

36501055

Title:

Puerariae lobatae Radix Alleviates Pre-Eclampsia by Remodeling Gut Microbiota and Protecting the Gut and Placental Barriers - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Neuroscience

Application:

IF:

3.7

Species:

Rattus norvegicus

PMID:

36720302

Title:

The role of CUL3 in cerebral ischemia-reperfusion injury

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

International wound journal

Application:

IF:

3.09

Species:

Mus musculus

PMID:

37042039

Title:

Edaravone promotes viability of random skin flaps via activating PI3K/Akt/mTOR signalling pathway‐mediated enhancement of autophagy

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Immunity, inflammation and disease

Application:

IF:

2.49

Species:

Rattus norvegicus

PMID:

37382268

Title:

Epimedium sagittatum Maxim ameliorates adriamycin-induced nephropathy by restraining inflammation and apoptosis via the PI3K/AKT signaling pathway - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Mus musculus

PMID:

37630322

Title:

Cepharanthine Exerts Antioxidant and Anti-Inflammatory Effects in Lipopolysaccharide (LPS)-Induced Macrophages and DSS-Induced Colitis Mice - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

5.56

Species:

Mus musculus

PMID:

37893693

Title:

Inhibition of IEC-6 Cell Proliferation and the Mechanism of Ulcerative Colitis in C57BL/6 Mice by Dandelion Root Polysaccharides - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

British Journal of Pharmacology

Application:

IF:

7.3

Species:

Rattus norvegicus

PMID:

37485568

Title:

Inhibition of Brd4 alleviates osteoarthritis pain via suppression of neuroinflammation and activation of Nrf2mediated antioxidant signaling

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Animal Nutrition

Application:

IF:

6.3

Species:

Sus scrofa

PMID:

38023380

Title:

Replacing dietary sodium selenite with biogenic selenium nanoparticles improves the growth performance and gut health of early-weaned piglets

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

International journal of biological macromolecules

Application:

IF:

8.02

Species:

Bos taurus

PMID:

36243161

Title:

Lentinan inhibits oxidative stress and alleviates LPS-induced inflammation and apoptosis of BMECs by activating the Nrf2 signaling pathway

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

Application:

IF:

2.24

Species:

Mus musculus

PMID:

37357660

Title:

Autophagy gene Atg7 regulates the development of radiationinduced skin injury and fibrosis of skin

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

The Science of the total environment

Application:

IF:

9.8

Species:

Mus musculus

PMID:

38369134

Title:

MAPK/NF-B signaling mediates atrazine-induced cardiorenal syndrome and antagonism of lycopene

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Microorganisms

Application:

IF:

4.5

Species:

Mus musculus

PMID:

38399751

Title:

Syringaldehyde Exhibits Antibacterial and Antioxidant Activities against Mycobacterium marinum Infection - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.3

Species:

Mus musculus

PMID:

38506087

Title:

4-octyl itaconate alleviates cisplatin-induced ferroptosis possibly via activating the NRF2/HO-1 signalling pathway - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Molecular biology reports

Application:

IF:

2.74

Species:

Mus musculus

PMID:

38270817

Title:

Sodium humate ameliorates LPS-induced liver injury in mice by inhibiting TLR4/NF-B and activating NRF2/HO-1 signaling pathways

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Molecular nutrition & food research

Application:

IF:

6.57

Species:

Mus musculus

PMID:

36738079

Title:

Oxidative Stress Mediated by N6Methyladenosine Methylation Contributes to HighFat Diet Induced Male Reproductive Dysfunction

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Rattus norvegicus

PMID:

36156620

Title:

Carvacrol preserves antioxidant status and attenuates kidney fibrosis via modulation of the TGF-1/Smad signaling and inflammation

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

34494061

Title:

The preventive effect and underlying mechanism of Rhus chinensis Mill. fruits on dextran sulphate sodium-induced ulcerative colitis in mice

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Life sciences

Application:

IF:

6.78

Species:

Rattus norvegicus

PMID:

35378138

Title:

Biochanin A alleviates unilateral ureteral obstruction-induced renal interstitial fibrosis and inflammation by inhibiting the TGF-1/Smad2/3 and NF-kB/NLRP3 signaling axis in mice

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Rattus norvegicus

PMID:

35687823

Title:

Rosmarinic Acid Inhibits Platelet Aggregation and Neointimal Hyperplasia In Vivo and Vascular Smooth Muscle Cell Dedifferentiation, Proliferation, and Migration In

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Rattus norvegicus

PMID:

35157955

Title:

Rhus chinensis Mill. fruits prevent necrotizing enterocolitis in rat pups via regulating the expressions of key proteins involved in multiple signaling pathways

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Toxicology

Application:

IF:

4.57

Species:

Homo sapiens

PMID:

37419272

Title:

Resveratrol protects against deoxynivalenol-induced ferroptosis in HepG2 cells

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Mus musculus

PMID:

31814398

Title:

Lycopene prevents DEHP-induced Leydig cell damage with the Nrf2 antioxidant signaling pathway in mice

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38570541

Title:

Nobiletin alleviates cisplatin-induced ototoxicity via activating autophagy and inhibiting NRF2/GPX4-mediated ferroptosis

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Mus musculus

PMID:

38064762

Title:

Shank3 ameliorates neuronal injury after cerebral ischemia/reperfusion via inhibiting oxidative stress and inflammation

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

RT-qPCR, WB

IF:

7.41

Species:

Homo sapiens

PMID:

36965257

Title:

Melatonin alleviates doxorubicin-induced cardiotoxicity via inhibiting oxidative stress, pyroptosis and apoptosis by activating Sirt1/Nrf2 pathway

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

6.1

Species:

Mus musculus

PMID:

38492240

Title:

Orientin Promotes Antioxidant Capacity, Mitochondrial Biogenesis, and Fiber Transformation in Skeletal Muscles through the AMPK Pathway

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38744845

Title:

CBX7 silencing promoted liver regeneration by interacting with BMI1 and activating the Nrf2/ARE signaling pathway - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

7.9

Species:

Mus musculus

PMID:

38833791

Title:

Targeting antioxidant factor Nrf2 by raffinose ameliorates lipid dysmetabolism-induced pyroptosis, inflammation and fibrosis in NAFLD

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Neuroreport

Application:

IF:

1.7

Species:

Rattus norvegicus

PMID:

38968575

Title:

Propofol ameliorates cognitive deficits following splenectomy in aged rats by inhibiting ferroptosis via the SIRT1/Nrf2/GPX4 pathway

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Journal of Zhejiang University. Science. B

Application:

IF:

5.1

Species:

Mus musculus

PMID:

38910496

Title:

Heme oxygenase 1 linked to inactivation of subchondral osteoclasts in osteoarthritis - PMC

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

8.9

Species:

Mus musculus

PMID:

38423272

Title:

Inhalation exposure to polystyrene nanoplastics induces chronic obstructive pulmonary disease-like lung injury in mice through multi-dimensional assessment

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Carbohydrate polymers

Application:

IF:

11.2

Species:

Homo sapiens

PMID:

38616079

Title:

Structure elucidation and antiviral activity of a cold water-extracted mannogalactofucan Ts1-1A from Trametes sanguinea against human cytomegalovirus in vitro

References for [KD Validated] NQO1 Rabbit mAb (A19586)

Product:

[KD Validated] NQO1 Rabbit mAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

38373191