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[KD Validated] TGF beta 1 Rabbit mAb (A25313)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from wild type (WT) and TGF beta 1 knockdown (KD) 293T cells using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KD Validated] TGF beta 1 Rabbit mAb (A25313)

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Overview

Product name	[KD Validated] TGF beta 1 Rabbit mAb
Catalog No.	A25313
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC3248

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Immunogen information

Immunogen	Recombinant protein
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	CED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta; TGF-beta1; TGF beta 1
Calculated MW	44kDa
Observed MW	12kDa/44kDa/12kDa, 44kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	A549, K-562, Rat spleen, 293T
Cellular location	Secreted, extracellular matrix, extracellular space.
Customer validation	IHC(Rattus norvegicus) WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] TGF beta 1 Rabbit mAb images

Western blot - [KD Validated] TGF beta 1 Rabbit mAb (A25313)

Immunohistochemistry - [KD Validated] TGF beta 1 Rabbit mAb (A25313)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KD Validated] TGF beta 1 Rabbit mAb (A25313)

Product:

[KD Validated] TGF beta 1 Rabbit mAb

Journal:

Molecular medicine reports

Application:

IHC

IF:

3.4

Species:

Rattus norvegicus

PMID:

38639180

Title:

Dicoumarol attenuates NLRP3 inflammasome activation to inhibit inflammation and fibrosis in knee osteoarthritis - PMC

References for [KD Validated] TGF beta 1 Rabbit mAb (A25313)

Product:

[KD Validated] TGF beta 1 Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Mus musculus

PMID:

39341804

Title:

Ternary inulin hydrogel with long-term intestinal retention for simultaneously reversing IBD and its fibrotic complication - PMC

References for [KD Validated] TGF beta 1 Rabbit mAb (A25313)

Product:

[KD Validated] TGF beta 1 Rabbit mAb

Journal:

Journal of clinical periodontology

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

38379293

Title:

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

References for [KD Validated] TGF beta 1 Rabbit mAb (A25313)

Product:

[KD Validated] TGF beta 1 Rabbit mAb

Journal:

International wound journal

Application:

IF:

3.1

Species:

Homo sapiens

PMID:

39379061

Title:

Combination therapy of negative pressure wound therapy and antibiotic-loaded bone cement for accelerating diabetic foot ulcer healing: A prospective randomised controlled trial - PMC

References for [KD Validated] TGF beta 1 Rabbit mAb (A25313)

Product:

[KD Validated] TGF beta 1 Rabbit mAb

Journal:

iScience

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

39252968