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[KO Validated] ALDH3A2 Rabbit pAb (A19899)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] ALDH3A2 Rabbit pAb (A19899)

Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit pAb (A19899) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name[KO Validated] ALDH3A2 Rabbit pAb
Catalog No.A19899
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Mutations in the gene cause Sjogren-Larsson syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 250-320 of human ALDH3A2 (NP_001026976.1).
SequenceEASLQNQIVWKIKETVKEFYGENIKESPDYERIINLRHFKRILSLLEGQKIAFGGETDEATRYIAPTVLTD
Gene ID
Swiss Prot
SynonymsSLS; FALDH; ALDH10; A2
Calculated MW55kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationCytoplasmic side, Endoplasmic reticulum membrane, Single-pass membrane protein
ABclonal:Western blot - [KO Validated] ALDH3A2 Rabbit pAb (A19899)}

Western blot - [KO Validated] ALDH3A2 Rabbit pAb (A19899)

Western blot analysis of lysates from wild type (WT) and ALDH3A2 knockout (KO) HeLa cells, using [KO Validated] ALDH3A2 Rabbit pAb (A19899) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)