제품 > 항체 > 다중클론항체(pAb)

[KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from wild type (WT) and BAF57/SMARCE1 knockout (KO) 293T cells, using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353)

Immunofluorescence analysis of HeLa cells using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

ABclonal:Immunoprecipitation - [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353)

Immunoprecipitation analysis of 150 μg extracts of Jurkat cells using 3 μg BAF57/BAF57/SMARCE1 antibody (A13353). Western blot was performed from the immunoprecipitate using BAF57/BAF57/SMARCE1 antibody (A13353) at a dilution of 1:1000.

Overview

Product name	[KO Validated] BAF57/SMARCE1 Rabbit pAb
Catalog No.	A13353
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human BAF57/BAF57/SMARCE1 (NP_003070.3).
Sequence	MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE
Gene ID
Swiss Prot
Synonyms	CSS5; BAF57; E1
Calculated MW	47kDa
Observed MW	50kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T
Cellular location	Nucleus
Customer validation	WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구후성유전학 & 핵 신호전달염색질 리모델링

[KO Validated] BAF57/SMARCE1 Rabbit pAb images

Western blot - [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353)

Immunofluorescence - [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353)

Immunofluorescence analysis of HeLa cells using [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Immunoprecipitation - [KO Validated] BAF57/SMARCE1 Rabbit pAb (A13353)

* For research use only. Not for therapeutic or diagnostic purposes.