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[KO Validated] Bax Rabbit pAb (A0207)

KO/KDValidated

Reviews (6)Publications (253) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A0207)

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A0207)

Western blot analysis of various lysates using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A0207)

Western blot analysis of lysates from Mouse brain , using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A0207)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of HeLa cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of HepG2 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of MCF7 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A0207)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A0207)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A0207)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A0207)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A0207)
Review
ABclonal: review for [KO Validated] Bax Rabbit pAb(A0207)

Overview

Product name[KO Validated] Bax Rabbit pAb
Catalog No.A0207
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL . In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion . The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C.BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human bax gene has been shown to be directly regulated by p53, and the bax promoter contains four motifs with homology to consensus p53-binding sites. Furthermore, p53 directly interacts with BAX to promote its activation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
SequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Gene ID
Swiss Prot
SynonymsBCL2 Associated X; Bcl-2-Like Protein 4; Bcl2-L-4; BCL2L4; BAX
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHT-1080, Raji, 293T
Cellular locationCytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein.
Customer validation

(Eoma cell, Human liver cell, Human pancreatic cancer cells, Human skin T cell lymphoma, Hut102, HCT116)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Gallus gallus, Giardia duodenalis, Capra hircus, Ctenopharyngodon, Ctenopharyngodon idellus, quails, Mus musculus、Sus scrofa, Coturnix japonica, Bos taurus, Homo sapiens, Eriocheir sinensis, Mus musculus, Rattus norvegicus, Felis catus, Oryctolagus cuniculus, Lampetra japonica, Other)

IF(Rattus norvegicus, Mus musculus)

IF(Homo sapiens)

IHC(Homo sapiens, Mus musculus, Gallus gallus)

IP(Homo sapiens, Mus musculus)

IHC(Homo sapiens, Mus musculus, Rattus norvegicus)

RT-PCR(Homo sapiens)

FC(Bos taurus)

IP(Mus musculus)

WB(Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A0207)}

Western blot - [KO Validated] Bax Rabbit pAb (A0207)

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A0207)}

Western blot - [KO Validated] Bax Rabbit pAb (A0207)

Western blot analysis of various lysates using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KO Validated] Bax Rabbit pAb (A0207)}

Western blot - [KO Validated] Bax Rabbit pAb (A0207)

Western blot analysis of lysates from Mouse brain , using [KO Validated] Bax Rabbit pAb (A0207) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A0207)}

Immunohistochemistry - [KO Validated] Bax Rabbit pAb (A0207)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)}

Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of HeLa cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)}

Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of HepG2 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)}

Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of MCF7 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)}

Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)}

Immunofluorescence - [KO Validated] Bax Rabbit pAb (A0207)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Bax Rabbit pAb (A0207) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0207

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Reviews List

Publishing research using A0207? Please let us know so that we can cite the reference in this datasheet.

항체 (5)

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WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

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WBIHC-PIF/ICCIPELISA

Reactivity:Human, Mouse, Rat

[KO Validated] Bax Rabbit mAb

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WBIF/ICCIPELISA

Reactivity:Human, Mouse, Rat

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WBIF/ICCELISA

Reactivity:Human, Mouse, Rat

Bax Rabbit pAb

ELISA 키트 (1)

Human Bcl2 Associated X Protein ELISA Kit (Bax)
RK00968

Human Bcl2 Associated X Protein ELISA Kit (Bax)

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)