제품 > 항체 > 다중클론항체(pAb)

[KO Validated] CD44 Rabbit pAb (A12410)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using [KO Validated] CD44 Rabbit pAb (A12410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from wild type (WT) and CD44 knockout (KO) HeLa cells, using [KO Validated] CD44 Rabbit pAb (A12410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] CD44 Rabbit pAb (A12410)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of A431 cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of RAW264.7 cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	[KO Validated] CD44 Rabbit pAb
Catalog No.	A12410
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-178 of human CD44 (NP_000601.3).
Sequence	AQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDV
Gene ID
Swiss Prot
Synonyms	IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; H-CAM; HCELL; ECM-III; HUTCH-1; HUTCH-I; ECMR-III; Hermes-1; 44
Calculated MW	82kDa
Observed MW	80kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, HUVEC, Mouse spleen
Cellular location	Cell membrane, Single-pass type I membrane protein
Customer validation	FC(Homo sapiens) IF(Mus musculus, Homo sapiens, Rattus norvegicus) WB(Homo sapiens, Mus musculus, Bos taurus) IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] CD44 Rabbit pAb images

Western blot - [KO Validated] CD44 Rabbit pAb (A12410)

Immunohistochemistry - [KO Validated] CD44 Rabbit pAb (A12410)

Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Confocal immunofluorescence analysis of A431 cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Immunofluorescence analysis of RAW264.7 cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Confocal immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

International journal of physiology, pathophysiology and pharmacology

Application:

IF:

2.997

Species:

Homo sapiens

PMID:

31333810

Title:

The effect of Korean red ginseng on mesenchymal stem cells from healthy and osteoporotic human bone marrow.

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Biochem Biophys Res Commun

Application:

IF:

3.575

Species:

Mus musculus

PMID:

32828281

Title:

The microgravity induces the ciliary shortening and an increased ratio of anterograde/retrograde intraflagellar transport of osteocytes

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Ann Clin Lab Sci

Application:

IF:

1.25

Species:

Homo sapiens

PMID:

33653777

Title:

All-Trans Retinoic Acid Potentiates Antitumor Efficacy of Cisplatin by Increasing Differentiation of Cancer Stem-Like Cells in Cervical Cancer

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Aging

Application:

IF:

5.95

Species:

Homo sapiens

PMID:

36367777

Title:

MAGE-A3 regulates tumor stemness in gastric cancer through the PI3K/AKT pathway - PMC

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Cancer cell international

Application:

IHC

IF:

6.42

Species:

Homo sapiens

PMID:

37158903

Title:

Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis - PMC

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Cancer cell international

Application:

IHC

IF:

6.42

Species:

Homo sapiens

PMID:

37158903

Title:

Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis - PMC

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Discover. Oncology

Application:

IF:

Species:

Mus musculus

PMID:

37639070

Title:

Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells - PMC

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Veterinary microbiology

Application:

IF:

3.24

Species:

Bos taurus

PMID:

36502739

Title:

Phospholipase C-1 potentially facilitates subcellular localization of activated -catenin, p--catenin (S552), during bovine herpesvirus 1 productive infection

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Chemosphere

Application:

IF:

8.94

Species:

Rattus norvegicus

PMID:

38158086

Title:

Interacting with luteinizing hormone receptor provides a new elucidation of the mechanism of anti-androgenicity of bisphenol S

References for [KO Validated] CD44 Rabbit pAb (A12410)

Product:

[KO Validated] CD44 Rabbit pAb

Journal:

Journal of biomedical science

Application:

IF:

Species:

Mus musculus

PMID:

38790021

Title:

Tumor necrosis factor-inducible gene 6 protein and its derived peptide ameliorate liver fibrosis by repressing CD44 activation in mice with alcohol-related liver disease - PMC