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[KO Validated] CDK2 Rabbit pAb (A0294)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using [KO Validated] CDK2 Rabbit pAb (A0294) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

Western blot analysis of lysates from wild type(WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit pAb (A0294) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] CDK2 Rabbit pAb (A0294)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using [KO Validated] CDK2 Rabbit pAb (A0294) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Review

Overview

Product name	[KO Validated] CDK2 Rabbit pAb
Catalog No.	A0294
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 200-298 of human CDK2 (NP_001789.2).
Sequence	RALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
Gene ID
Swiss Prot
Synonyms	CDKN2; p33(CDK2); K2
Calculated MW	34kDa
Observed MW	34kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293T, HeLa
Cellular location	Cajal body, Cytoplasm, Endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Customer validation	(Human colon cancer cell, Mouse kidney) WB(Homo sapiens, Rattus norvegicus, Mus musculus, Bos taurus, Mus musculus, ) FC(Bos taurus) IHC(Homo sapiens, Mus musculus,) IHC(Homo sapiens) WB(Homo sapiens, Mus musculus) Co-IP(Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] CDK2 Rabbit pAb images

Western blot - [KO Validated] CDK2 Rabbit pAb (A0294)

Immunohistochemistry - [KO Validated] CDK2 Rabbit pAb (A0294)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0294

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Reviews List

Product Protocols

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Molecules

Application:

IF:

4.411

Species:

Homo sapiens

PMID:

28098804

Title:

GADD45a Regulates Olaquindox-Induced DNA Damage and S-Phase Arrest in Human Hepatoma G2 Cells via JNK/p38 Pathways

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

4.432

Species:

Rattus norvegicus

PMID:

31103630

Title:

Simvastatin inhibits the proliferation of HL-60 clone 15- derived eosinophils by inducing the arrest of the cell cycle in the G1/S phase.

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Cell Cycle

Application:

IF:

4.534

Species:

Mus musculus

PMID:

32816597

Title:

The PRC2 complex directly regulates the cell cycle and controls proliferation in skeletal muscle

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Molecular therapy. Nucleic Acids

Application:

IF:

8.886

Species:

Mus musculus

PMID:

32059342

Title:

Upregulation of lncRNA LINC00460 Facilitates GC Progression through Epigenetically Silencing CCNG2 by EZH2/LSD1 and Indicates Poor Outcomes

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Cell Biol Toxicol

Application:

IF:

6.691

Species:

Homo sapiens

PMID:

33126266

Title:

Induction of cell cycle arrest and apoptosis by CPUC002 through stabilization of p53 and suppression of STAT3 signaling pathway in multiple myeloma

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Biochem Pharmacol

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

34111425

Title:

Inhibition of Pim-2 kinase by LT-171-861 promotes DNA damage and exhibits enhanced lethal effects with PARP Inhibitor in multiple myeloma

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Rattus norvegicus

PMID:

36228355

Title:

Decabromodiphenyl ethane induces male reproductive toxicity by glycolipid metabolism imbalance and meiotic failure

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Rattus norvegicus

PMID:

35955918

Title:

Lidocaine Inhibited Tendon Cell Proliferation and Extracellular Matrix Production by Down Regulation of Cyclin A, CDK2, Type I and Type III Collagen Expression - PMC

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

ACS applied bio materials

Application:

IF:

Species:

Homo sapiens

PMID:

35005910

Title:

A Lipidated Peptide with Mitochondrial Membrane Localization in Human A549 Lung Cells: From Enhanced Cell-Penetrating Properties to Biological Activity Mechanism

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

IF:

2.65

Species:

Homo sapiens

PMID:

35911149

Title:

Arisaema heterophyllum Blume Monomer Stigmasterol Targets PPARγ and Inhibits the Viability and Tumorigenicity of Lung Adenocarcinoma Cells NCI-H1975 - PMC

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Molecular neurobiology

Application:

IF:

5.68

Species:

Homo sapiens

PMID:

36053436

Title:

Isobavachalcone's Alleviation of Pyroptosis Contributes to Enhanced Apoptosis in Glioblastoma: Possible Involvement of NLRP3

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

35526567

Title:

Sampsonione F suppresses adipogenesis via activating p53 pathway during the mitotic clonal expansion progression of adipocyte differentiation

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Cell biology international

Application:

IF:

4.47

Species:

Homo sapiens

PMID:

36153645

Title:

RAB3D, upregulated by aryl hydrocarbon receptor (AhR), promotes the progression of prostate cancer by activating the PI3K/AKT signaling pathway

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Biological & pharmaceutical bulletin

Application:

IF:

2.26

Species:

Homo sapiens

PMID:

36450527

Title:

TLR9 Exerts an Oncogenic Role in Promoting Osteosarcoma Progression Depending on the Regulation of NF-κB Signaling Pathway

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

4.13

Species:

Mus musculus

PMID:

36660952

Title:

Promoting effects of calponin 3 on the growth of diffuse large B-cell lymphoma cells - PMC

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Journal of hazardous materials

Application:

IF:

14.22

Species:

Mus musculus

PMID:

36493639

Title:

Prenatal and Postnatal Exposure to Polystyrene Microplastics Induces Testis Developmental Disorder and Affects Male Fertility in Mice

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

The Journal of nutritional biochemistry

Application:

IF:

6.11

Species:

Mus musculus

PMID:

37023933

Title:

Ketogenic Diet Alleviates Renal Interstitial Fibrosis in UUO Mice by Regulating Macrophage Proliferation

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Cell reports

Application:

IF:

Species:

Homo sapiens

PMID:

36995937

Title:

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Oncology letters

Application:

IF:

3.11

Species:

Homo sapiens

PMID:

37113396

Title:

Inhibitory effect and mechanism of hirsuteine on NCI-H1299 lung cancer cell lines - PMC

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Experimental and therapeutic medicine

Application:

IF:

2.75

Species:

Homo sapiens

PMID:

37123214

Title:

PKNOX2 suppresses lung cancer cell proliferation by inhibiting the PI3K/AKT/mTOR axis - PMC

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

37485365

Title:

Daughter cell fate choice instructed preemptively by mother cells facing nutrient limitation - PMC

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Journal of cellular physiology

Application:

IF:

6.51

Species:

Mus musculus

PMID:

37218742

Title:

miR‐136‐5p/FZD4 axis is critical for Wnt signaling‐mediated myogenesis and skeletal muscle regeneration

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Toxicology and applied pharmacology

Application:

IF:

4.46

Species:

Mus musculus

PMID:

37783235

Title:

Di-(2-ethylhexyl) phthalate aggravates psoriasis-like skin lesions: In vitro and in vivo evaluation

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Journal of medicinal chemistry

Application:

IF:

8.03

Species:

Homo sapiens

PMID:

37671907

Title:

Degradation of Cyclin-Dependent Kinase 9/Cyclin T1 by Optimized Microtubule-Associated Protein 1 Light Chain 3 Beta-Recruiting Coumarin Analogs

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Experimental cell research

Application:

IF:

4.14

Species:

Homo sapiens

PMID:

35405117

Title:

Deubiquitinating enzyme PSMD14 facilitates gastric carcinogenesis through stabilizing PTBP1

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Immunology

Application:

IF:

7.21

Species:

Homo sapiens

PMID:

38361445

Title:

NK92 cells and peripheral blood NK cells respond oppositely upon dasatinib treatment

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

4.13

Species:

Homo sapiens

PMID:

38186304

Title:

CDC6 may serve as an indicator of lung adenocarcinoma prognosis and progression based on TCGA and GEO data mining and experimental analyses

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Platelets

Application:

IF:

4.23

Species:

Homo sapiens

PMID:

27780401

Title:

Platelet rich plasma releasate promotes proliferation of skeletal muscle cells in association with upregulation of PCNA, cyclins and cyclin dependent kinases

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

J Exp Clin Cancer Res

Application:

IF:

11.161

Species:

Mus musculus

PMID:

34886901

Title:

ZIP10 is a negative determinant for anti-tumor effect of mannose in thyroid cancer by activating phosphate mannose isomerase

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Animal biotechnology

Application:

WB, FC

IF:

2.14

Species:

Bos taurus

PMID:

37022008

Title:

CircRRAS2 promotes myogenic differentiation of bovine MuSCs and is a novel regulatory molecule of muscle development

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

European journal of medicinal chemistry

Application:

WB, IHC

IF:

7.08

Species:

Homo sapiens, Mus musculus,

PMID:

37837671

Title:

Design, synthesis, and biological evaluation of novel 4, 4-bipyridine derivatives acting as CDK9-Cyclin T1 protein-protein interaction inhibitors against triple

References for [KO Validated] CDK2 Rabbit pAb (A0294)

Product:

[KO Validated] CDK2 Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Mus musculus

PMID:

37923710