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[KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

KO/KDValidated

Review (1)Publications (46) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Western blot analysis of lysates from wild type(WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Review
ABclonal: review for [KO Validated] CDKN1A/p21 Rabbit mAb(A19094)

Overview

Product name[KO Validated] CDKN1A/p21 Rabbit mAb
Catalog No.A19094
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51034
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1).
SequenceMSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP
Gene ID
Swiss Prot
SynonymsP21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; 21
Calculated MW18kDa
Observed MW18kDa(Mouse)/21kDa(Human/Rat)
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7 cells, HeLa cells, 293T cells, Mouse large intestine, Mouse testis, Rat lung
Cellular locationCytoplasm, Nucleus.
Customer validation

(MDA-MB-231 cells)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Mus musculus , Oryctolagus cuniculus, Mus musculus)

IHC(Homo sapiens, Rattus norvegicus)

IF(Homo sapiens, Rattus norvegicus)

IF(Homo sapiens, Mus musculus, Mus musculus , Rattus norvegicus)

IHC(Homo sapiens, Rattus norvegicus)

WB(Homo sapiens)

RT-qPCR(Homo sapiens)

PCR(Homo sapiens,Mus musculus)

qPCR(Homo sapiens,Mus musculus)

RT-qPCR(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Western blot - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Western blot analysis of lysates from wild type(WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)}

Immunohistochemistry - [KO Validated] CDKN1A/p21 Rabbit mAb (A19094)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)