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[KO Validated] β-Catenin Rabbit mAb (A19657)

KO/KDValidated

Review (1)Publications (83) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from HeLa cells, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from Mouse brain, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from wild type (WT) and β-Catenin knockout (KO) 293T cells, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from NIH/3T3 cells, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from wild type(WT) and β-Catenin knockout (KO) 293T(KO) cells, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human solitary fibrous tumor tissue using [KO Validated] β-Catenin Rabbit mAb (A19657) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Confocal imaging of paraffin-embedded Human prostate cancer tissue using [KO Validated] β-Catenin Rabbit mAb (A19657, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] β-Catenin Rabbit mAb (A19657, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] β-Catenin Rabbit mAb (A19657, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] β-Catenin Rabbit mAb (A19657, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg β-Catenin antibody (A19657). Western blot was performed from the immunoprecipitate using β-Catenin (A19657) at a dilution of 1:1000.

Review
ABclonal: review for [KO Validated] β-Catenin Rabbit mAb(A19657)

Overview

Product name[KO Validated] β-Catenin Rabbit mAb
Catalog No.A19657
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0136
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (P35222).
SequenceMATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP
Gene ID
Swiss Prot
SynonymsEVR7; CTNNB; MRD19; NEDSDV; armadillo; in
Calculated MW85kDa
Observed MW92kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa cells, 293T cells, NIH/3T3 cells, Mouse brain
Cellular locationCell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Customer validation

(normal mouse hippocampus)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Mus musculus , Mus musculus, Gallus gallus)

IHC(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Homo sapiens, Mus musculus, Oryctolagus cuniculus)

IP(Homo sapiens)

WB(Mus musculus)

IHC(Mus musculus, Rattus norvegicus)

RT-qPCR(Rattus norvegicus)

Co-IP(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Mus musculus,Homo sapiens)

qPCR(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from HeLa cells, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from Mouse brain, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from wild type (WT) and β-Catenin knockout (KO) 293T cells, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from NIH/3T3 cells, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of lysates from wild type(WT) and β-Catenin knockout (KO) 293T(KO) cells, using [KO Validated] β-Catenin Rabbit mAb (A19657) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry analysis of paraffin-embedded Human solitary fibrous tumor tissue using [KO Validated] β-Catenin Rabbit mAb (A19657) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Confocal imaging of paraffin-embedded Human prostate cancer tissue using [KO Validated] β-Catenin Rabbit mAb (A19657, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] β-Catenin Rabbit mAb (A19657, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] β-Catenin Rabbit mAb (A19657, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] β-Catenin Rabbit mAb (A19657, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg β-Catenin antibody (A19657). Western blot was performed from the immunoprecipitate using β-Catenin (A19657) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (13)

재조합 단백질 (1)

ELISA 키트 (1)

Secondary Antibodies (26)