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[KO Validated] Claudin 1 Rabbit mAb (A21971)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KO Validated] Claudin 1 Rabbit mAb (A21971) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from wild type(WT) and Claudin 1 knockdown (KD) Hep G2 cells, using [KO Validated] Claudin 1 Rabbit mAb (A21971) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	[KO Validated] Claudin 1 Rabbit mAb
Catalog No.	A21971
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC54475

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 112-211 of human Claudin 1 (NP_066924.1).
Sequence	EVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
Gene ID
Swiss Prot
Synonyms	CLD1; SEMP1; ILVASC; 1
Calculated MW	23kDa
Observed MW	20kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:12000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431, Hep G2, Mouse liver, Mouse kidney, Rat kidney
Cellular location	Cell junction, Cell membrane, Multi-pass membrane protein, tight junction.
Customer validation	WB(Sus scrofa, Mus musculus, Anatinae, Coturnix japonica, Homo sapiens, Capra hircus, Rattus norvegicus) IHC(Mus musculus) IF(Sus scrofa, Mus musculus, Homo sapiens) WB(Mus musculus) IF(Mus musculus) IHC(Mus musculus) WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구세포 생물학 & 발달 생물학세포 접착밀착연접

[KO Validated] Claudin 1 Rabbit mAb images

Western blot - [KO Validated] Claudin 1 Rabbit mAb (A21971)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

7.31

Species:

Sus scrofa

PMID:

36035212

Title:

Biogenic Selenium Nanoparticles Alleviate Intestinal Epithelial Barrier Damage through Regulating Endoplasmic Reticulum Stress-Mediated Mitophagy - PMC

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IHC

IF:

5.19

Species:

Mus musculus

PMID:

36030029

Title:

Glycyrrhiza uralensis fisch. Alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal …

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Applied and environmental microbiology

Application:

IF:

Species:

Mus musculus

PMID:

34524898

Title:

Protective Effects of Selenium Nanoparticle-Enriched Lactococcus lactis NZ9000 against Enterotoxigenic Escherichia coli K88-Induced Intestinal Barrier Damage in Mice

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Anatinae

PMID:

35935959

Title:

Hexavalent Chromium Exposure Induces Intestinal Barrier Damage via Activation of the NF-κB Signaling Pathway and NLRP3 Inflammasome in Ducks - PMC

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

The Journal of nutrition

Application:

IF:

4.68

Species:

Sus scrofa

PMID:

36084016

Title:

Lycopene Affects Intestinal Barrier Function and the Gut Microbiota in Weaned Piglets Via Antioxidant Signaling Regulation

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Journal of hazardous materials

Application:

IF:

14.22

Species:

Coturnix japonica

PMID:

36209609

Title:

Effects of thiacloprid exposure on microbiota–gut–liver axis: Multiomics mechanistic analysis in Japanese quails

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.67

Species:

Sus scrofa

PMID:

36290726

Title:

Acidifiers Attenuate Diquat-Induced Oxidative Stress and Inflammatory Responses by Regulating NF-κB/MAPK/COX-2 Pathways in IPEC-J2 Cells - PMC

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Homo sapiens

PMID:

37180168

Title:

CD73 mediated host purinergic metabolism in intestine contributes to the therapeutic efficacy of a novel mesenchymal-like endometrial regenerative cells against experimental colitis - PMC

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Veterinary microbiology

Application:

IF:

3.24

Species:

Sus scrofa

PMID:

37068404

Title:

PRRSV-induced inflammation in pulmonary intravascular macrophages (PIMs) and pulmonary alveolar macrophages (PAMs) contributes to endothelial barrier …

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Toxicology

Application:

IF:

4.57

Species:

Homo sapiens

PMID:

37442268

Title:

Selenium nanoparticles alleviate deoxynivalenol-induced intestinal epithelial barrier dysfunction by regulating endoplasmic reticulum stress in IPEC-J2 cells

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

IF, WB

IF:

3.32

Species:

Mus musculus

PMID:

37979398

Title:

NCOA4-mediated ferritinophagy aggravate intestinal oxidative stress and ferroptosis after traumatic brain injury

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

International journal of biological macromolecules

Application:

IF:

8.2

Species:

Mus musculus

PMID:

38307433

Title:

Oral delivery of miR-146a-5p overexpression plasmid-loaded Pickering double emulsion modulates intestinal inflammation and the gut microbe

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Molecules (Basel, Switzerland)

Application:

WB, IF

IF:

4.92

Species:

Mus musculus

PMID:

36144494

Title:

Recombinant Human Annexin A5 Alleviated Traumatic-Brain-Injury Induced Intestinal Injury by Regulating the Nrf2/HO-1/HMGB1 Pathway - PMC

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Food & function

Application:

WB, IHC

IF:

6.31

Species:

Mus musculus

PMID:

35527507

Title:

Polysaccharides from soybean residue fermented by Neurospora crassa alleviate DSS-induced gut barrier damage and microbiota disturbance in mice

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Cells

Application:

IF:

7.66

Species:

Sus scrofa

PMID:

36611975

Title:

CXCR3 Inhibition Blocks the NF-B Signaling Pathway by Elevating Autophagy to Ameliorate Lipopolysaccharide-Induced Intestinal Dysfunction in Mice - PMC

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Toxicology

Application:

IF:

4.57

Species:

Mus musculus

PMID:

37075930

Title:

Polystyrene microplastics aggravate acute pancreatitis in mice

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Animals : an open access journal from MDPI

Application:

IF:

3.23

Species:

Capra hircus

PMID:

35681868

Title:

Regulation of Tight Junctions by Sex Hormones in Goat Mammary Epithelial Cells

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

6.2

Species:

Rattus norvegicus

PMID:

38880160

Title:

Identification and experimental validation of immune-related gene PPARG is involved in ulcerative colitis

References for [KO Validated] Claudin 1 Rabbit mAb (A21971)

Product:

[KO Validated] Claudin 1 Rabbit mAb

Journal:

International journal of nanomedicine

Application:

IF, WB

IF:

Species:

Homo sapiens

PMID:

39371479

Title:

Tangerine Peel-Derived Exosome-Like Nanovesicles Alleviate Hepatic Steatosis Induced by Type 2 Diabetes: Evidenced by Regulating Lipid Metabolism and Intestinal Microflora - PMC