제품 > 항체 > 단일클론항체(mAb)

[KO Validated] Cyclin B1 Rabbit mAb (A19037)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit mAb (A19037) at 1:5000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Western blot analysis of lysates from Jurkat cells using [KO Validated] Cyclin B1 Rabbit mAb (A19037) at 1:5000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product name	[KO Validated] Cyclin B1 Rabbit mAb
Catalog No.	A19037
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0474

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635).
Sequence	LRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAY
Gene ID
Swiss Prot
Synonyms	CCNB; B1
Calculated MW	48kDa
Observed MW	55kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2500 - 1:10000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, Jurkat
Cellular location	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus, Other) IHC(Homo sapiens, Mus musculus) IF(Homo sapiens, Mus musculus) IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Cyclin B1 Rabbit mAb images

Western blot - [KO Validated] Cyclin B1 Rabbit mAb (A19037)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Journal of cancer research and clinical oncology

Application:

IF:

4.32

Species:

Homo sapiens

PMID:

36471019

Title:

Orelabrutinib and venetoclax synergistically induce cell death in double-hit lymphoma by interfering with the crosstalk between the PI3K/AKT and p38/MAPK signaling

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

World journal of surgical oncology

Application:

IF:

3.25

Species:

Homo sapiens

PMID:

36907878

Title:

Homeobox D9 drives the malignant phenotypes and enhances the Programmed death ligand-1 expression in non-small cell lung cancer cells via binding to Angiopoietin-2 promoter - PMC

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

JCI insight

Application:

IF:

9.48

Species:

Homo sapiens

PMID:

37288663

Title:

RNF112-mediated FOXM1 ubiquitination suppresses the proliferation and invasion of gastric cancer - PMC

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

J Int Med Res

Application:

IHC, IF

IF:

1.573

Species:

Homo sapiens, Mus musculus

PMID:

34044639

Title:

Cyclin B1 acts as a tumor microenvironment-related cancer promoter and prognostic biomarker in hepatocellular carcinoma

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Environmental science and pollution research international

Application:

IF:

5.19

Species:

Homo sapiens

PMID:

37037937

Title:

BPA induces placental trophoblast proliferation inhibition and fetal growth restriction by inhibiting the expression of SRB1

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Medical oncology (Northwood, London, England)

Application:

IF:

3.73

Species:

Homo sapiens, Mus musculus

PMID:

37634215

Title:

EZH2 inhibitor Tazemetostat synergizes with JQ-1 in esophageal cancer by inhibiting c-Myc signaling pathway

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Communications biology

Application:

IF:

6.54

Species:

Homo sapiens

PMID:

38337031

Title:

Functional analysis of Cdc20 reveals a critical role of CRY box in mitotic checkpoint signaling - PMC

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Aging

Application:

IF:

5.95

Species:

Homo sapiens

PMID:

38244585

Title:

SLC7A8 overexpression inhibits the growth and metastasis of lung adenocarcinoma and is correlated with a dismal prognosis - PMC

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Mus musculus

PMID:

38424044

Title:

MYC induces CDK4/6 inhibitors resistance by promoting pRB1 degradation - PMC

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Platelets

Application:

IF:

4.23

Species:

Homo sapiens

PMID:

27780401

Title:

Platelet rich plasma releasate promotes proliferation of skeletal muscle cells in association with upregulation of PCNA, cyclins and cyclin dependent kinases

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

37009226

Title:

Copper overload impairs hematopoietic stem and progenitor cell proliferation via prompting HSF1/SP1 aggregation and the subsequently downregulating FOXM1-Cytoskeleton axis - PMC

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Genes & genomics

Application:

IF:

2.16

Species:

Homo sapiens

PMID:

36346541

Title:

Guanine nucleotide-binding protein 2, GNBP2, accelerates the progression of clear cell renal cell carcinoma via regulation of STAT3 signaling transduction pathway

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Acta biomaterialia

Application:

IF:

10.63

Species:

Mus musculus

PMID:

36549633

Title:

Codelivery of adavosertib and olaparib by tumor-targeting nanoparticles for augmented efficacy and reduced toxicity

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Journal of agricultural and food chemistry

Application:

WB, IHC, IF

IF:

5.89

Species:

Mus musculus

PMID:

37811833

Title:

Vitisin A, as a Type of Pyranoanthocyanin, Suppresses Inflammation by Restricting Hematopoietic Stem Cell Differentiation toward Monocytes in Bone Marrow

References for [KO Validated] Cyclin B1 Rabbit mAb (A19037)

Product:

[KO Validated] Cyclin B1 Rabbit mAb

Journal:

Application:

IF:

Species:

Rattus norvegicus

PMID:

38726683

Title:

The in vitro mechanism of Vaspinregulates the proliferation and steroidogenesis of rat lutein cells