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[KO Validated] Cyclin D1 Rabbit mAb (A19038)

KO/KDValidated

Publications (108) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:[KO Validated] Cyclin D1 Rabbit mAb
ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of lysates from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of various lysates using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (A19038, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product name[KO Validated] Cyclin D1 Rabbit mAb
Catalog No.A19038
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0300
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).
SequenceISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
Gene ID
Swiss Prot
SynonymsBCL1; PRAD1; U21B31; D11S287E; D1
Calculated MW34kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:100 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2 , MCF7 , HeLa , Mouse lung
Cellular locationNucleus.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Homo sapeins)

IF(Mus musculus, Rattus norvegicus, Homo sapiens)

IHC(Mus musculus)

Co-IP(Homo sapiens)

IHC(Homo sapiens)

RT-qPCR(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)}

Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of lysates from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)}

Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of various lysates using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)}

Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (A19038, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19038? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)