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[KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Western blot analysis of various lysates, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (FASN) antibody (A22462PM) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Western blot analysis of lysates from wild type(WT) and Fatty Acid Synthase (FASN) knockout (KO) HeLa cells, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Immunofluorescence analysis of HeLa cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Immunofluorescence analysis of HepG2 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb®
Catalog No.A22462PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) Rabbit PolymAb® (NP_004095.4).
SequenceQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG
Gene ID
Swiss Prot
SynonymsFAS; OA-519; SDR27X1
Calculated MW273kDa
Observed MW273kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IF/ICC 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293F, NIH/3T3, Mouse liver, HeLa
Cellular locationCytoplasm, Melanosome.
ABclonal:Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)}

Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Western blot analysis of various lysates, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (FASN) antibody (A22462PM) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)}

Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Western blot analysis of lysates from wild type(WT) and Fatty Acid Synthase (FASN) knockout (KO) HeLa cells, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)}

Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Immunofluorescence analysis of HeLa cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)}

Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Immunofluorescence analysis of HepG2 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)}

Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)}

Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM)

Immunofluorescence analysis of PC-12 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

ELISA 키트 (1)

Secondary Antibodies (26)