Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182)
Western blot analysis of lysates from 293F cells, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182) at1:1800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182)
Western blot analysis of lysates from Rat liver, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182) at1:1800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot - [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182)
Western blot analysis of lysates from wild type (WT) and Fatty Acid Synthase (FASN) knockout (KO) HeLa cells, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182) at1:1800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182)
Confocal imaging of PC-12 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182)
Confocal imaging of Hep G2 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182)
Confocal imaging of NIH/3T3 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182)
Confocal imaging of HeLa cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb (A21182, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.