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[KO Validated] HMGB1 Rabbit mAb (A19529)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529)

Western blot analysis of various lysates using [KO Validated] HMGB1 Rabbit mAb (A19529) at 1:6000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

Western blot analysis of lysates from wild type (WT) and HMGB1 knockout (KO) HeLa cells using [KO Validated] HMGB1 Rabbit mAb (A19529) at 1:6000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] HMGB1 Rabbit mAb (A19529) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse colon using [KO Validated] HMGB1 Rabbit mAb (A19529) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] HMGB1 Rabbit mAb (A19529) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using [KO Validated] HMGB1 Rabbit mAb (A19529) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KO Validated] HMGB1 Rabbit mAb (A19529) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver using [KO Validated] HMGB1 Rabbit mAb (A19529) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] HMGB1 Rabbit mAb (A19529)

Confocal imaging of NIH-3T3 cells using [KO Validated] HMGB1 Rabbit mAb (A19529, dilution 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product name	[KO Validated] HMGB1 Rabbit mAb
Catalog No.	A19529
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0001

Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (P09429).
Sequence	SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEE
Gene ID
Swiss Prot
Synonyms	HMG1; HMG3; HMG-1; SBP-1; B1
Calculated MW	25kDa
Observed MW	25kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:6000 - 1:60000 IHC-P 1:200 - 1:2000 IF/ICC 1:100 - 1:800 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293F, HeLa, MCF7, Mouse brain, Mouse liver, Rat liver
Cellular location	Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted.
Customer validation	IF(Rattus norvegicus, Mus musculus) IHC(Rattus norvegicus, Mus musculus ) WB(Bos taurus, Mus musculus, Homo sapiens, Mus musculus) IHC(Mus musculus) WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] HMGB1 Rabbit mAb images

Western blot - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunohistochemistry - [KO Validated] HMGB1 Rabbit mAb (A19529)

Immunofluorescence - [KO Validated] HMGB1 Rabbit mAb (A19529)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] HMGB1 Rabbit mAb (A19529)

Product:

[KO Validated] HMGB1 Rabbit mAb

Journal:

J Leukoc Biol

Application:

IF:

4.96

Species:

Rattus norvegicus

PMID:

34402106

Title:

High mobility group box-1 serves a pathogenic role in spinal cord injury via the promotion of pro-inflammatory cytokines

References for [KO Validated] HMGB1 Rabbit mAb (A19529)

Product:

[KO Validated] HMGB1 Rabbit mAb

Journal:

Life sciences

Application:

IHC

IF:

6.78

Species:

Rattus norvegicus

PMID:

36209830

Title:

Agmatine-mediated inhibition of NMDA receptor expression and amelioration of dyskinesia via activation of Nrf2 and suppression of HMGB1/RAGE/TLR4/MYD88/NF …

References for [KO Validated] HMGB1 Rabbit mAb (A19529)

Product:

[KO Validated] HMGB1 Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

17.52

Species:

Mus musculus

PMID:

36494092

Title:

Peptide Supramolecular Assembly‐Instructed In Situ Self‐Aggregation for Stratified Targeting Sonodynamic Therapy Enhancement of AIE Luminogens

References for [KO Validated] HMGB1 Rabbit mAb (A19529)

Product:

[KO Validated] HMGB1 Rabbit mAb

Journal:

International immunopharmacology

Application:

IHC

IF:

5.71

Species:

Rattus norvegicus

PMID:

37012873

Title:

Cilostazol novel neuroprotective mechanism against rotenone-induced Parkinson's disease in rats: Correlation between Nrf2 and HMGB1/TLR4/PI3K/Akt/mTOR …

References for [KO Validated] HMGB1 Rabbit mAb (A19529)

Product:

[KO Validated] HMGB1 Rabbit mAb

Journal:

International journal of oral science

Application:

IHC

IF:

24.89

Species:

Mus musculus

PMID:

37532700

Title:

Role of dendritic cells in MYD88-mediated immune recognition and osteoinduction initiated by the implantation of biomaterials - PMC

References for [KO Validated] HMGB1 Rabbit mAb (A19529)

Product:

[KO Validated] HMGB1 Rabbit mAb

Journal:

Virulence

Application:

IF:

5.42

Species:

Bos taurus

PMID:

34008469

Title:

-catenin has potential effects on the expression, subcellular localization, and release of high mobility group box 1 during bovine herpesvirus 1 productive infection in MDBK cell culture

References for [KO Validated] HMGB1 Rabbit mAb (A19529)

Product:

[KO Validated] HMGB1 Rabbit mAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF, IHC, WB

IF:

6.63

Species:

Mus musculus

PMID: