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[KO Validated] IFITM3 Rabbit pAb (A13070)

KO/KDValidated

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)

Western blot analysis of lysates from HeLa cells, using [KO Validated] IFITM3 Rabbit pAb (A13070) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)

Western blot analysis of lysates from wild type (WT) and IFITM3 knockout (KO) HeLa cells, using [KO Validated] IFITM3 Rabbit pAb (A13070) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of C6 cells using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of L929 cells using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of U-2 OS cells using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] IFITM3 Rabbit pAb
Catalog No.A13070
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Interferon-induced transmembrane (IFITM) proteins are a family of interferon induced antiviral proteins. The family contains five members, including IFITM1, IFITM2 and IFITM3 and belong to the CD225 superfamily. The protein encoded by this gene restricts cellular entry by diverse viral pathogens, such as influenza A virus, Ebola virus and Sars-CoV-2.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human IFITM3 (NP_066362.2).
SequenceMNHTVQTFFSPVNSGQPPNYEMLKEEHEVAVLGAPHNPAPPTSTVIHIRSETSVPDHVVWSLFNTLFMNPCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGILMTILLIVIPVLIFQAYG
Gene ID
Swiss Prot
Synonyms1-8U; IP15; DSPA2b; M3
Calculated MW15kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCell membrane, Late endosome membrane, Lysosome membrane, Single-pass type II membrane protein
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)}

Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)

Western blot analysis of lysates from HeLa cells, using [KO Validated] IFITM3 Rabbit pAb (A13070) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)}

Western blot - [KO Validated] IFITM3 Rabbit pAb (A13070)

Western blot analysis of lysates from wild type (WT) and IFITM3 knockout (KO) HeLa cells, using [KO Validated] IFITM3 Rabbit pAb (A13070) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] IFITM3 Rabbit pAb (A13070)}

Immunohistochemistry - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)}

Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of C6 cells using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)}

Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of L929 cells using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)}

Immunofluorescence - [KO Validated] IFITM3 Rabbit pAb (A13070)

Immunofluorescence analysis of U-2 OS cells using [KO Validated] IFITM3 Rabbit pAb (A13070) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13070? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)